J4 ›› 2013, Vol. 12 ›› Issue (9): 17-20.DOI: 10. 3969/j. issn. 1672-2345. 2

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Pharmacokinetics Parameters of Ferulic Acid Methylester Nitric Oxide Donor in Mice

  

  1. 1. College of Pharmacy and Chemistry, Dali University, Dali，Yunnan 671000, China;2. College of Chemical and Chemistry Engineering, Hunan University, Changsha 410082, China
  • Received:2013-06-07 Revised:2013-06-21 Online:2013-09-15 Published:2013-09-15

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Abstract:

Objective: To develop the assay method by RP-HPLC for ferulic acid methylester nitric oxide donor （FMND）and
determine the pharmacokinetics parameters in mice after oral administration. Methods: The sample was extracted by acetoacetate,
followed by vortex and high speed centrifugation, dried by nitrogen flow and dissolved by mobile phase before HPLC analysis. The
HPLC condition was applied with mobile phase at methanol: water （70:30）, flow rate at 1.0 mL/min, column temperature at 30 0C.
Results: FMND was absorbed quickly with a lag time of 6.6 min after oral administration in mice at the dose of 50 mg/kg, and
arrived to the peak concentration at about 1.5 h. The distribution in vivo matched two-compartment model with a lag time. The
distribute half life time（t1/2α）was 1.04 h, the eliminate half life time（t1/2 β）was 9.44 h, the absorb half life time（t1/2 Ka）was 0.94 h.
Conclusion: FMND was absorbed quickly in mice after oral administration, and the distribute equilibrium arrived moderately with a
long eliminate half life time.

Key words: 阿魏酸, 一氧化氮供体, 高效液相色谱, 药动学

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