J4 ›› 2014, Vol. 13 ›› Issue (4): 25-29.

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Expressions and Clinical-pathological Significance of TGF-β1,Caspase-3 and WT1 in Glomerulonephritis

  

  1. Affiliated Hospital of Dali University,Dali,Yunnan 671000,China
  • Received:2013-10-11 Revised:2014-01-06 Online:2014-04-15 Published:2014-04-15

Abstract:

Objective:To explore the expression and significance of transforming growth factor-β1(TGF-β1),cysteinyl aspartate specific proteinase -3 (Caspase -3)and Wilms' tumor suppressor gene 1 (WT1)in renal biopsy tissues of glomerulonephritis.Methods:40 cases of renal biopsy tissues and 7 cases of control renal tissues were detected and analyzed by Envision immunohistochemistry. Results:TGF -β1,Caspase -3 and WT1 expressed in all cases of renal tissues. There were significant differences between each trail grouping and normal control group (P<0.05). The positive retes of TGF-β1 in group of mesangial proliferative glomerulonephritis were lower than membranous nephropathy(t=-4.131,P=0.002). Expression of Caspase-3 showed no differences in divided groups. The expression of WT1 in group of massive proteinuria were lower than that non-massive proteinuria
(t =-2.130,P =0.046). Other designed groups have no significant difference. Meanwhile,higher WT1 expression showed the correlation with lower expression of TGF-β1 and Caspase-3. Conclusion:Up-regulated expression of TGF-β1 and Caspase-3 and down-regulated expression of WT1 were closely related to pathologic change in kidney. Proteinuria and podcytes loss is probably the predictor of renal disease progression and a cause for glomerulosclerosis.

Key words: renal biopsy, TGF-β1, Caspase-3, WT1, proteinuria, immunohistochemistry

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