J4 ›› 2014, Vol. 13 ›› Issue (8): 18-22.

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The Study of Auto-inducing Fermentation of Mycobacterium tuberculosis Protein MTB8.1

  

  1. 1. Pre-clinical College, Dali University, Dali,Yunnan 671000,China; 2. SHKB Pharmaceutical Co., Ltd., Beijing 100070, China
  • Received:2014-02-24 Online:2014-08-15 Published:2014-08-15

Abstract:

Objective: To explore an effective fermentation process of Mycobacterium tuberculosis protein MTB8.1 with soluble
expression, which would be suitable for industrial development. Methods: Strain pUC18/MTB8.1/DH10B was used as fermentation strain and a systematic study of the composition of medium and the parameters of fermentation was conducted to obtain a new fermentation process. Results: By means of the new fermentation process, the yield of soluble recombinant protein MTB8.1 was greatly increased in per unit volume of fermentation broth, with about 30 mg of soluble protein obtained from every liter of fermentation broth through purification. Conclusion: A better way of soluble expression of MTB8.1 in Escherichia coli expression system was found tentatively, which laid the theoretical foundation for the industrial production.

Key words: Mycobacterium tuberculosis, protein MTB8.1, soluble expression, fermentation process, antigenicity

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