EZH2 Protein Expression Changes in the Neuron Devlopment

Abstract

Abstract:

Objective: To study the role of histone methyltransferase EZH2 in the neuron development. Methods: Mouse embryonic
stem cells were induced into neural cells by all-trans retinoic acid（RA）in vitro, which was verified by cell morphology and real-time
quantitative RT- PCR. EZH2 protein expression level during the differentiation procedure was evaluated by Western blot assays.
Results: In 5 days of E14 embryonic stem cells differentiation, the protein expression levels of EZH2 were the highest in the state of
embryonic stem cells and 1d after induction, the protein expression levels then declined rapidly. At the end of the 5d, protein expression
level was the lowest. Conclusion: This research observed the specific expression change of EZH2 protein in the differentiation of neuron,
which is the foundation for further study of EZH2.

Key words: EZH2, embryonic stem cells, neuron

CLC Number:

R329.2

Sun Fang, Huan Tong, Pan Yun, Li Yan. EZH2 Protein Expression Changes in the Neuron Devlopment[J]. J4, 2015, 14(10): 9-12.

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