Abstract:

Objective: To optimize extraction technology of total flavonoids from the flower of Juglans sigillata Dode and establish its
content determination method. Methods: On the basis of single factor test，orthogonal experiment was used to to optimize extraction
technology of total flavonoids from the flower of Juglans sigillata Dode. With Rutin as the reference substance, differential
spectrophotometry method was adopted to determine the content of total flavonids of the flower of Juglans sigillata Dode. Results: The
optimum extraction conditions were described as follows: ethanol volume scores 50%, the solid / liquid ratio 1：20, 3 times of heating
reflux，and the time duration for each extraction was 40 min. Total flavonoids of the flower of Juglans sigillata Dode showed good linear
relationship in the range of 11~77 μg/mL（r =0.999 3），the average recovery was 99.7%，RSD=1.94%. The content of total flavonoids
was 2.24%. Conclusion: The method was simple，accurate，stable and reliable, and could be used to measure the content of total
flavones of the flower of Juglans sigillata Dode.

Key words: flower of Juglans sigillata Dode, differential spectrophotometry, total flavonoids, content determination