Damage Resistance of Basal Forebrain Nestin-positive Cholinergic Neurons

Abstract

Abstract:

Objective: To explore the different responses of basal forebrain nestin positive and negative cholinergic neurons to the
neurotoxicity. Methods: 30 SD rats were randomly divided into 5 groups, which are control group and colchicine Ⅰ,Ⅱ,Ⅲ,Ⅳ groups
（10, 20, 50, 100 μg）. 10 microliters of normal saline or different doses of colchicine were injectedintracerebroventricularly. 24 hours
after injection, 4% paraformaldehyde intracardiac perfusion, brain removal, frozen section, choline acetyltransferase and nestin
immunohistochemistry were carried out. Then, the nestin positive and negative cholinergic neurons of different basal forebrain regions
were counted and analyzed. Results: After intracerebroventricular injection of colchicine, the nestin positive and negative cholinergic
neurons in different regions of rats' basal forebrain decreased. The greater the colchicine injection dose is, the more obvious the
decrease of the neurons. Compared with nestin positive neurons, the nestin negative cholinergic neurons decreased earlier and more
significantly. Conclusion: the nestin expression can enhance the damage resistant ability of basal forebrain cholinergic neurons.

Key words: basal forebrain, nestin, cholinergic neurons, neural plasticity

CLC Number:

R322.81

Wang Huanhuan, Zhu Jianhua. Damage Resistance of Basal Forebrain Nestin-positive Cholinergic Neurons[J]. J4, 2016, 1(8): 35-38.

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