Abstract:

Objective: To develop a method for revising and improving the quality standards of the Qingfei Yihuo Tablet. Methods:
Microscopic identification and TLC method were adopted to identify the quality of the Dahuang, Jugeng, Huangbo and Kusheng in
preparation and analyzing the Huangqigan in Huangqi by the method of HPLC. The analysis was performed on an Agilent TC-C18
column with the mobile phase consisting of methanol（A）−0.4% phosphate acid（53：47）by gradient elution at a flow rate of 0.6
mL/min. The detection wavelength was set at 280 nm and the column temperature was 30 ℃. Results: Calcium oxalate crystal clusters
were checked out by the method of microscopic identification, the diameter of Dahuang was 60-140 microns and the shape of Jugeng
was inulin fan or quasi-circular. Additional, Dahuang, Huangbo and Kusheng were detected out by the methods of TLC and the HPLC
results showed that the linear ranges were 0.025-4.000 μg/mL（r=0.999 8）for Huangqigan. The average recoveries（n=9）were
99.8%, RSD=0.34%. Conclusion: The developed method is accurate, reproducible and specific and the quality of the preparation can
be controlled.

Key words: Qingfei Yihuo Tablet, microscopic identification, TLC, HPLC, Huangqigan