›› 2017, Vol. 2 ›› Issue (8): 6-9.
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Objective:To investigate in vitro antibacterial activity of the alcohol extracts and extract fractions of Rubus delavayi. Methods:5 extract fractions were obtained through 95% ethanol reflux by extracting Rubus delavayi. The minimum inhibitory concentration(MIC), the minimum bactericidal concentration(MBC)and the inhibition zone of the crude extract and 5 extract fractions were determined by tube dilution and disk diffusion method. The antibacterial activity of Rubus delavayi was observed. Results:The alcohol extracts, its ethyl acetate fraction and its N-butanol fraction of Rubus delavayi showed high antibacterial activity against eight tested bacteria; lower antioxidant activity was observed in the water fraction; the chloroform fraction had a significantly inhibitory effect on Staphylococcus aureus, Proteusbacillus species, Bacillus subtilis and Salmonella typhi; the petroleum ether fraction had a certain inhibition on Staphylococcus aureus and Bacillus subtilis. Conclusion: The alcohol extracts and its different extract fractions of Rubus delavayi have in vitro antibacterial activity against almost all the studied bacteria.
Key words: Rubus delavayi, tube dilution, disk diffusion, in vitro antibacterial activity
CLC Number:
R285
Liu Xiaobo, Guo Meixian, Shi Guirong, Guo Yuqin. Study on in Vitro Antibacterial Activity of Rubus delavayi[J]. , 2017, 2(8): 6-9.
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http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/Y2017/V2/I8/6
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