›› 2018, Vol. 3 ›› Issue (10): 22-25.

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Effects of 28 Species of AM Fungi on Diosgenin Contents in Paris polyphylla var. yunnanensis

  

  1. (1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2. College of Food and Biological Engineering,
    Chongqing Three Gorges University, Chongqing 404120, China; 3. The Chongqing Engineering Laboratory for Green Cultivation and
    Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs, Chongqing 404120, China; 4.Editorial Department of Journal,
    Dali University, Dali, Yunnan 671003, China)
  • Received:2018-07-10 Revised:2018-10-13 Online:2018-10-15 Published:2018-10-15

Abstract:

〔Abstract〕Objective: After 28 foreign species of AM fungi were inoculated in sterilized soil, the effects of the AM on mycorrhizal
colonization and the diosgenin content of Paris polyphylla var. yunnanensis were observed by inoculation test in pot at room
temperature. Methods: Trypan blue staining was used to test AM colonization, and HPLC was applied for analyzing diosgenin content.
Results: The good symbiotic relationship between Paris polyphylla var. yunnanensis and AM fungi species was observed, and AM fungi
species enhanced the colonization. Most AM fungi species did not influence the diosgenin content(0.978 2~10.745 3 mg/g)compared
with the control group(CK). However, Svi fungi significantly increased the diosgenin content(41.404 5 mg/g). Conclusion: There are
symbiotic relationships between arbuscular mycorrhizal(AM)fungi and Paris polyphylla var. yunnanensis. AM fungi species can
enhance the colonization, and Svi fungi species can greatly increase the diosgenin content.

Key words: 〔Key words〕Paris polyphylla var. yunnanensis, AM fungi, colonization, diosgenin

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