Abstract:

〔Abstract〕Objective: To study the effect of Kangfuxin liquid on gut flora in rats with ulcerative colitis induced by 2，4-
dinitrochlorobenzene combined with acetic acid. Methods: Rat models of UC were established by using DNCB combined with acetic
acid. Groups of UC were treated with Mesalazine, and low, medium and high doses of Kangfuxin respectively. Normal saline was given
to the control group and the model group. After their final treatment rats were sacrificed, and fecal samples were collected.
Fluorescence quantitative PCR was used to detect the changes in the number of major bacterial species in fecal samples of rats in each
group. Results: Compared with the model group, the number of Bacteroides and Bifidobacterium increased in all the groups treated with
Kangfuxin. The number of Lactobacillus increased and the number of Escherichia coli significantly reduced in low-dose Kangfuxin
group; Enterococcus significantly decreased in medium-dose and high-dose Kangfuxin groups（P<0.05）. Conclusion: Kangfuxin
liquid can improve people's gut bacteria and help control ulcerative colitis by increasing the number of probiotics, reducing the number
of opportunistic pathogens and balancing gut flora.

Key words: 〔Key words〕Kangfuxin liquid, ulcerative colitis, 2, 4-dinitrochlorobenzene, gut flora, Fluorescence quantitative PCR detection