Effects of the 7-Hydroxyisoflavone in the Proliferative Activity of HaCaT Cells and UVB-induced Photodamage

Abstract

Abstract:

〔Abstract〕 Objective： To investigate the effects of the 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells and the UVB-induced photodamage， as well as its mechanism. Methods： The cell counting assay was used to detect the effects of different concentrations of 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells. Next， the HaCat cells were pretreated with 200 μmol/L 7-Hydroxyisoflavone and were exposed to UVB（30 mJ/cm2）. Then， the production of reactive oxygen species（ROS） was detected by fluorescence method. The expressions of PARP1 and Caspase-8 were detected by Western blot. Results：The 7-Hydroxyisoflavone had the most obvious effect on the proliferative activity of HaCaT cells at the concentration of 200 μmol/L， and it could reduce the production of UVB-induced ROS. Besides， it could also inhibit the expressions of the PARP1 and Caspase-8.Conclusion：The 7-Hydroxyisoflavone can reduce photodamage caused by UVB and has a photoprotective effect on cells， which can be used to prevent skin damage caused by UVB.

Key words:

"> ">〔Key words">〕 7-Hydroxyisoflavone">, UVB">, HaCaT cells">, reactive oxygen species">, apoptosis

CLC Number:

Duan Yi, Ni Changwen, Wang Minhua, Sun Sujiao, Huang Ling.

Effects of the 7-Hydroxyisoflavone in the Proliferative Activity of HaCaT Cells and UVB-induced Photodamage [J]. Journal of Dali University, 2022, 7(4): 34-39.

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