Journal of Dali University ›› 2022, Vol. 7 ›› Issue (6): 97-100.

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Effectiveness Evaluation of an Improved Method for Soil Nematodes Isolation

  

  1. 1. Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China;2. College of Agriculture and Biology Science, Dali University, Dali, Yunnan 671003, China
  • Received:2021-05-20 Revised:2021-06-16 Online:2022-06-15 Published:2022-07-04

Abstract:

Objective To evaluate the further improved versionimproved method 2 of improved Baermann funnel method improved method 1 reported in the literature. Methods Soil artificially added with quantitative nematodes and natural farmland soil were used as experimental materials and then the isolation rate of soil nematodes and costs of shallow dish method Baermann funnel method improved method 1 improved method 2 and LUDOX TM suspension method were measured. Results The isolation rate of nematodes by the improved method 2 showed no significant difference from that by LUDOX TM suspension method but is significantly higher than those by other methodsP<0.01. Conclusion Improved method 2 has obvious advantages in terms of nematode isolation rate operation time and cost.

Key words:

soil nematodes, Baermann funnel method, isolation rate, improvement

CLC Number: