Journal of Dali University ›› 2023, Vol. 8 ›› Issue (10): 21-26.

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The Inhibitory Activity of Pinus yunnanensis Pine Extract with Anti-HIV Activity against Human Coronavirus 229E

Li Yafei12Shang Fangjian12Luo Chunyu12Shi Zhefang3Liu Qi12*   

  1. 1.Pre-clinical CollegeDali UniversityDaliYunnan 671000China2.Yunnan Provincial Key Laboratory of Insect Biomedical Research and DevelopmentDaliYunnan 671000China3. Baoding Hospital of Beijing Children's HospitalCapital Medical UniversityBaodingHebei 071000China

  • Received:2023-03-20 Revised:2023-04-01 Online:2023-10-15 Published:2023-10-26

Abstract:

ObjectiveTo evaluate the inhibitory activity of Pinus yunnanensis pine extractreferred to as "pine extract"with anti-HIV activity against human coronavirus HCoV-229E and preliminarily determine its target. MethodsA cell-cell fusion model and  model mediated by HCoV-229E S protein were constructed to detect the inhibitory activity of pine extract against HCoV-229E. Time-of-addition and time-of-removal experiments were performed to determine the target and the timing of action. ResultsPine extract could inhibit the cell-cell fusion mediated by HCoV-229E S proteinwith an IC50 of 0.136±0.010mg/mL. It effectively inhibited HCoV-229E infectionwith an IC50 of 0.069±0.015mg/mL. Time-of-removal and time-of-addition experiments showed that pine extract targeted the HCoV-229E S protein and acted in the early stage of infection1 h before); different concentrations of pine tower extracts have no significant toxicity to 293T and Huh-7 cells. ConclusionPine extract can target the HCoV-229E S protein and inhibit HCoV-229E infection in the early stagemaking it a safe candidate drug.

Key words:

Pinus yunnanensis, pine extract, HCoV-229E, inhibitory activity

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