Abstract: Objective: To study the physiological and biochemical index and accumulation of secondary metabolites in the callus of
Pueraria mirifica affected by the different levels of nitrogen（ N） and kalium（ K）. Methods: Physiological, biochemical and accumulation
of secondary metabolites in the callus of P. mirifica under different treatment conditions were studied by means of plant physiology
experiment and HPLC. Results: The contents of puerarin, daidzin and genistein in the callus of P. mirifica were negatively correlated
with the concentrations of NH4NO3 and KNO3 （P<0.01）. The synthesis of puerarin was most favored when the concentrations of
NH4NO3 and KNO3 were 1 650.0 mg/L and 0.0 mg/L, respectively. The synthesis of genistein was most favored when the concentrations of
NH4NO3 and KNO3 were 825.0 mg/L and 950.0 mg/L, respectively. When the concentrations of NH4NO3 and KNO3 were 0.0 mg/L and 1 900.0
mg/L, respectively, it was most favorable for the synthesis of daidzin and total isoflavones. The content of geranylgeranyl was negatively
correlated with the activities of catalase （CAT） and superoxide dismutase （SOD） （P<0.05）. The content of daidzin was positively
correlated with the content of malondialdehyde（ MDA）（ P<0.01）. The content of genistein was positively correlated with MDA content
and peroxidase （POD） activity （P<0.05）. Conclusion: The synthesis of puerarin, daidzin and genistein, MDA content, SOD activity,
POD activity and CAT activity in the callus of P. mirifica are affected by different levels of N and K.

Key words: Pueraria mirifica, callus, high performance liquid chromatography, secondary metabolites, tissue culture