Table of Content

15 August 2024, Volume 9 Issue 8

Previous Issue   

Evaluation of Taste and Screening of Proprietary Sweeteners for 25 Bitter Chinese Herbs#br# Commonly Used in Clinic

Lu Tai, Yang Xin, Lin Junzhi, Qiu Min, Zhang Dingkun,

2024, 9(8):  1-9.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 001

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Objective: To explore the real taste characteristics and the application rules of sweeteners for 25 bitter Chinese herbs
commonly used in clinic. Methods: The volunteer visual analogue scoring method was used to classify the real taste of 25 kinds of
Chinese herbs, and grasp the main taste, taste grade, perception position and perception trend of each kind of Chinese herb. 11 kinds of
common sweeteners were selected, and the exclusive sweeteners that can effectively mask the bad taste of the above Chinese herbs were
matched through the volunteer tasting experiment. Results: The main tastes of different Chinese herbs were different. Huanglian and Longdan had intense bitterness, and Longdan had astringency; Huangbai and Chuanxinlian had strong bitterness, and Chuanxinlian had astringency; 13 herbs, such as Yanhusuo and Lianzixin, had obvious bitterness, and the aftertaste disappeared 3-4 minutes after spitting the liquid; Kuxingren and Yinchen had slight bitterness, and the aftertaste disappeared 1-3 minutes after spitting the liquid; Ganjiang had obvious spicy taste. The results of the specificity of sweeteners showed that the most suitable sweetener for Chuanxinlian was sucralose; for Longdan and Huangbai, it was neotame; for Huajuhong and Zhishi, it was mogroside; for Baibu and Zhebeimu, it was stevioside; for Lianqiao, it was aspartame; for Qinghao, it was glycyrrhizin; for Sanqi, it was acesulfame-K. Conclusion: The main taste of the above-mentioned Chinese herbs is bitter, and 3/5 of
them have concurrent tastes; the taste perception site of the vast majority of Chinese herbs is in the back of the mouth, and the spicy
taste perception is in the front of the tongue. According to the analysis of taste characteristics of 21 kinds of Chinese herbs, stevioside,
mogroside, sucralose and glycyrrhizin are suitable for suppressing the bitterness of most bitter Chinese herbs.

Study on Aromadendrane and Guaiane Sesquiterpenoids from the Rhizome of Dobinea delavayi

Zhao Xuan, , , Dong Litian, , , Chen Wei, , , Shen Yi, , , Li Gang, , , Xiao Chaojiang, , , Jiang Bei, ,

2024, 9(8):  10-13.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 002

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Objective: To gain a deeper understanding of the types and structures of sesquiterpenoids in the extract of Dobinea
delavayi, and to provide scientific basis for the development and utilization of this plant. Methods: Silica gel and Sephadex LH-20
column chromatography were used to separate and purify sesquiterpenoids from 80% ethanol extract of dried rhizome of D. delavayi,
and the compounds were structurally characterized on the basis of physicochemical properties and modern spectroscopic techniques.
Results: Six sesquiterpenoid compounds were isolated and identified as: 4β, 10β, 15-trihydroxy-aromadendrane-10, 15-acetonide
（1）, 4α-hydroxy-10β-methoxy-1β-H, 5β-H-aromadendrane （2）, 4β, 10β⁃aromadendranediol （3）, spathulenol （4）, 2-oxoguaia-1
（10）, 3, 5, 7（11）, 8-pentaen-12, 8-olide （5）, and taraxacin （6）. Conclusion: D. delavayi contains aromadendrane sesquiterpenoids
and guaiane sesquiterpenoids. This study provides a research basis for a deeper understanding of the chemical quality of D. delavayi.

Synthesis of the Natural Coumarin Products Murraol and （E）-Suberenol

Liu Zhiwen, , Gong Xushun, , Zhang Kai, , Lei Ting, , Jiang Shizhi,

2024, 9(8):  14-19.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 003

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Objective: To efficiently synthesize the natural coumarin products Murraol and （E）-Suberenol in a single step, and
provide strong support for the synthesis of similar natural products. Methods: Using brominated coumarin as the starting material, the
natural coumarin products Murraol and （E）-Suberenol were synthesized by Heck coupling reaction catalyzed by transition metal
palladium. Results: The synthesis of both natural coumarin products Murraol and （E）-Suberenol were efficiently and straightforwardly
achieved. Conclusion: The synthetic method has the characteristics of simple operation, mild reaction conditions and atomic economy,
and is suitable for industrial production.

Preparation of Lycopus lucidus Polysaccharide and Its Effects on High Glucose-Induced Rat Glomerular#br# Mesangial Cells

Qiu Jingyao, Wang Xin, Li Chunxu, Wang Fusheng

2024, 9(8):  20-25.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 004

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Objective: To investigate the preparation process of Lycopus lucidus polysaccharides （LLP） with different ethanol
precipitation fractions and evaluate their effects on high glucose-induced rat glomerular mesangial cells （HBZY-1）. Methods: The
crude LLP was obtained by deproteinization and macroporous resin column chromatography, and the components were obtained by
fractional ethanol precipitation. The activity of high glucose-induced HBZY-1 cells was tested in vitro. Results: Three different
ethanol precipitation fractions （LLP-30%, LLP-60%, and LLP-90%） showed no toxicity to normal HBZY-1 cells at concentrations
ranging from 0.25 to 4.00 mg/mL. LLP-60% and LLP-90% exhibited significant inhibitory effects on the proliferation of high glucoseinduced
HBZY-1 cells （P<0.01） and promoted the content of superoxide dismutase while reducing the contents of malondialdehyde
and reactive oxygen species. Conclusion: The extraction, separation and preparation process of LLP are feasible, and the active
component LLP-90% not only has the highest content, but also exhibits the most significant effects on inhibiting the proliferation of
high glucose-induced HBZY-1 cells and alleviating cellular oxidative stress, which provided the basis for the development of L.
lucidus as a functional food of diabetic nephropathy.

Physicochemical Properties of Methyl-p-Benzoquinone and Prediction of Its Drug Likeness

Shi Zhengmei, , , Wang Qi, , Lu Xixing, , Zeng Hongping, , Xiao Huai, , Zhang Chenggui, , , Liu Heng, ,

2024, 9(8):  26-32.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 005

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Objective: To determine the equilibrium solubility and oil-water partition coefficient of methyl-p-benzoquinone, and
analyze the cytotoxic activity against different tumor cells. Methods: The equilibrium solubility and oil-water partition coefficient of
methyl-p-benzoquinone in different pH solutions were determined by shake flask-HPLC method. Chromatographic conditions: Ecosil-
C18 chromatographic column（ 4.6 mm×250 mm, 5 μm）, the mobile phase was acetonitrile-0.1% formic acid aqueous solution（ 70 :30）,
the flow rate was 1.0 mL/min, the detection wavelength was 250 nm, the column temperature was 30 ℃, and the injection amount was
10 μL. SwissADME and pkCSM were used to predict the drug likeness. The cytotoxic activity of methyl-p-benzoquinone
against HT29, CT26, Caco-2, Hela and HepG2 cells was analyzed by MTT method. Results: The equilibrium solubility of methyl-pbenquinone
in solutions with pH of 1.2, 2.0, 5.0, 6.7, 7.0 and 7.4 for 12 h was 7.65, 8.05, 9.31, 8.43, 9.33 and 7.93 mg/mL,
respectively, and the oil-water partition coefficients were 0.78, 0.80, 0.83, 0.82, 0.77, 0.82, respectively. The solubility and oil-water
partition coefficient predicted by SwissADME were consistent with the experimental results. At the same time, the prediction results
showed that methyl-p-benzoquinone had better drug likeness and weaker blood-brain barrier permeability. The IC50 of methyl-pbenzoquinone
against 5 cell lines were 7.98, 3.21, 8.96, 7.96, 6.31 μmol/L, respectively. Conclusion: HPLC method is simple and
feasible. Moreover, the solubility of methyl-p-benzoquinone can be properly enhanced under the condition of pH 5.0~7.0 solution. At
the same time, the ADME/T online prediction software for drugs has certain reliability. Methyl p-benzoquinone has significant
inhibitory effect on multiple cell lines such as HT29 and CT26.

Multi-Index Optimization of Extraction Process for Hujiao-Biba by Box-Behnken Response Surface#br# Method Combined with BP Neural Network

Xiao Wanqing, Zou Chuncai, Yan Haiyan

2024, 9(8):  33-40.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 006

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Objective: To optimize the extraction process of Hujiao-Biba by using Box-Behnken response surface method combined
with BP neural network based on spectrum-effect relationship and entropy weight method. Methods: Effects of solid-liquid ratio,
extraction times and ultrasonic time on the ultrasonic extraction process of Hujiao-Biba were investigated by using Box-Behnken
response surface method, and the yield of dry extract and DPPH· clearance rate were calculated. HPLC fingerprint of Hujiao-Biba was
established and grey correlation method was used to determine the spectrum-effect relationship between fingerprint and DPPH·
clearance rate, and the correlation degree was calculated. The correlation degree was used to correct the peak area of piperine and the
total peak area of the fingerprint, and the corrected peak area was obtained. The weight of each evaluation index was assigned by
entropy weight method, and the comprehensive evaluation index was calculated. A BP neural network model was established to
optimize the experimental results and the optimal extraction process for Hujiao-Biba was ultimately obtained. Results: The optimal
extraction process for Hujiao-Biba obtained through Box-Behnken response surface method and BP neural network model prediction
was as follows: the solid-liquid ratio was 1 : 30 （g/mL）, the extraction times was 4 times and the ultrasonic time was 40 min. Conclusion: Box-Behnken response surface method combined with BP neural network model based on spectrum-effect relationship
and entropy weight method can be used to optimize the extraction process of Hujiao-Biba, laying a foundation for the preparation of
their extract and the study of related dosage forms.

Nondestructive Quality Evaluation of CⅡ- 3 Extract from Periplaneta americana Based on Infrared#br# Spectroscopy Combined with Chemometrics

Zhou Jiaojiao, Xia Conglong, Li Wencan, Wang Yanping, Li Wei, He Zhengchun

2024, 9(8):  41-45.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 007

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Objective: To establish a simple and nondestructive evaluation method of CⅡ-3 extract from Periplaneta americana
based on infrared spectrum and chemometrics. Methods: Infrared spectra of 10 batches of CⅡ-3 samples were established by infrared
spectroscopy, and the spectra were optimized by baseline correction, ordinate normalization and 15-point smoothing. Origin 9.1
software was used to characterize and analyze the spectral data, and the fingerprints of 10 batches of CⅡ-3 samples were established,
and similarity analysis and principal component analysis were performed. Results: The main characteristic peaks of the functional
group region in infrared spectra of 10 batches of CⅡ-3 samples were 3 300, 2 960, 2 930, 1 660, 1 630, 1 550, 1 450, 1 390 cm-1. The
main characteristic peaks in fingerprint region were 1 240, 1 080, 621, 546 cm-1. The peak shape, position and intensity of infrared
spectra were similar among different batches, and the correlation coefficients were all above 0.960. The results of principal component
analysis showed that 10 batches of samples were clustered into one category. Conclusion: The method has the advantages of simple
operations, strong specificity, no damage to the sample, and can be used for the quality evaluation for CⅡ-3 samples.

Determination of Related Substances in Hydrocortisone Butyrate Cream by HPLC

Rong Nana, Zhu Jialiang, Liu Jihua, Wang Ying, Wu Jing

2024, 9(8):  46-52.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 008

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Objective: To establish a method for the determination of related substances in hydrocortisone butyrate cream. Methods:
Kromasil XDB-C18（ 4.6 mm×250 mm, 5 μm） column was used for gradient elution with acetonitrile-0.5% glacial acetic acid solution as
the mobile phase. Detection was performed at a wavelength of 254 nm, and methodological validation was performed on the established
method. Results: Hydrocortisone butyrate was well separated from all impurities and had good linear relationship in the range of 0.05~
7.50 μg/mL. In all samples, hydrocortisone 21-butyrate was the highest single impurity, and the contents of hydrocortisone and other
unknown impurities were less than 0.26%, and the total impurities ranged from 0.37% to 2.72%. Under the condition of oxidation, high
temperature and strong light, hydrocortisone butyrate cream had good stability, and the impurity content did not increase significantly.
Under the condition of strong acid and strong base, hydrocortisone butyrate was easily degraded to produce hydrocortisone 21-butyrate.
Conclusion: The method established in this study has the advantages of good specificity, high sensitivity and low cost, which can
provide an important reference for the quality control of hydrocortisone butyrate cream.

Dosage Form Improvement and Quality Evaluation of the Kangfuxin Ye

Wang Chengjun, He Xu, Zhao Cuiyun, Deng Yeping

2024, 9(8):  53-56.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 009

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Objective: To change Kangfuxin Ye into tablets and the quality was evaluated. Methods: With hygroscopic capacity as
the index, L9 （34） orthogonal test was used to screen the best prescription of Kangfuxin tablets, and the quality was evaluated according
to Pharmacopoeia of the People's Republic of China （2020 edition）. Results: Kangfuxing tablets was composed of Periplaneta
americana extract and excipient （microcrystalline cellulose: lactose: hydroxypropyl methyl cellulose: microsilica gel = 3:2:1:0.5） in a
ratio of 1:3, and was prepared by wet granulation tablet method, and the quality met the relevant requirements. Conclusion: This
prescription has reasonable composition, simple preparation process, good stability and controllable quality.

Identification and Analysis of Polypeptides of Immunomodulatory Albumin from Zhiciweipi

Han Jun

2024, 9(8):  57-62.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 010

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Objective: To identify and analyze the polypeptides of immunomodulatory albumin（ ALB） from the Zhiciweipi. Methods: LCMS/
MS and bioinformatics software combined with homologous sequence comparison and evolutionary analysis were applied to
identifying the components after enzymolysis of Zhiciweipi and analyzing the immunomodulatory core proteins and peptides obtained
from the screening. Results: ALB was the key immunomodulatory protein and the active peptide protein sequence was
RHPYFYAPELLYF. Evolutionary tree analysis indicated that the ALB of Erinaceus europaeus was 100% homologous with that of
Elephantulus edwardii, with the closest genetic relationship. The homologous sequence comparison analysis showed that ALB of both
species contained 608 amino acids, with very similar amino acid sequence, and the active polypeptide protein sequence
RHPYFYAPELLYF had no difference. Conclusion: Polypeptides of ALB in Erinaceus europaeus and Elephantulus edwardii have similar
biological functions, and this provides part of the basic evidence for the clinical substitutability of rare animals.

Effects of Nitrogen and Kalium on Secondary Metabolites and Physiology and Biochemistry#br# in the Callus of Pueraria mirifica

Ma Shuyun, Xu Li

2024, 9(8):  63-66.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 011

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Objective: To study the physiological and biochemical index and accumulation of secondary metabolites in the callus of
Pueraria mirifica affected by the different levels of nitrogen（ N） and kalium（ K）. Methods: Physiological, biochemical and accumulation
of secondary metabolites in the callus of P. mirifica under different treatment conditions were studied by means of plant physiology
experiment and HPLC. Results: The contents of puerarin, daidzin and genistein in the callus of P. mirifica were negatively correlated
with the concentrations of NH4NO3 and KNO3 （P<0.01）. The synthesis of puerarin was most favored when the concentrations of
NH4NO3 and KNO3 were 1 650.0 mg/L and 0.0 mg/L, respectively. The synthesis of genistein was most favored when the concentrations of
NH4NO3 and KNO3 were 825.0 mg/L and 950.0 mg/L, respectively. When the concentrations of NH4NO3 and KNO3 were 0.0 mg/L and 1 900.0
mg/L, respectively, it was most favorable for the synthesis of daidzin and total isoflavones. The content of geranylgeranyl was negatively
correlated with the activities of catalase （CAT） and superoxide dismutase （SOD） （P<0.05）. The content of daidzin was positively
correlated with the content of malondialdehyde（ MDA）（ P<0.01）. The content of genistein was positively correlated with MDA content
and peroxidase （POD） activity （P<0.05）. Conclusion: The synthesis of puerarin, daidzin and genistein, MDA content, SOD activity,
POD activity and CAT activity in the callus of P. mirifica are affected by different levels of N and K.

Construction and Application Evaluation of Clinical Prediction Model for Type 2 Diabetes Patients#br# with Hyperuricemia

Zhou Ye, Fan Guanjie, Yang Bo, Zhang Hengyan, Yan Heguo,

2024, 9(8):  67-74.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 012

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Objective: To construct and evaluate a clinical prediction model for type 2 diabetes patients with hyperuricemia and
explore its independent risk factors. Methods: Retrospective analysis was conducted on the medical records of 553 type 2 diabetes
patients admitted to the department of endocrinology of Zhaotong Hospital of Traditional Chinese Medicine from January 2020 to
December 2021. The patients were divided into hyperuricemia group and non-hyperuricemia group according to whether hyperuricemia
was complicated or not. LASSO regression was used to select predictive variables and construct a Logistic regression model to identify
independent risk factors for type 2 diabetes patients with hyperuricemia. The model was visualized by drawing a nomogram, and the
predictive efficacy of the model was evaluated by the degree of differentiation, calibration and clinical applicability. Results: There
were statistically significant differences （P<0.05） between the two groups of patients in body mass index, concurrent diabetic
nephropathy, standardized hypoglycemic therapy, total cholesterol, triglycerides, serum creatinine, blood urea nitrogen, insulin
resistance index, dampness-heat obstructing the spleen syndrome, and qi-yin deficiency syndrome. Eight predictive variables were
selected by LASSO regression for multicollinearity diagnosis, and the prediction model was constructed after the qi-yin deficiency
syndrome was deleted and multi-factor Logistic regression was performed. The results showed that seven predictive variables were
independent risk factors for type 2 diabetes patients with hyperuricemia （P<0.05）, and the model had good differentiation, calibration
and clinical applicability. Conclusion: Body mass index, concurrent diabetic nephropathy, standardized hypoglycemic therapy, total
cholesterol, triglycerides, insulin resistance index, and dampness-heat obstructing the spleen syndrome are independent risk factors for
type 2 diabetes patients with hyperuricemia. The clinical prediction model based on these factors can provide a reliable basis for the
clinical prevention and treatment of hyperuricemia in patients with type 2 diabetes.

KAP Investigation and Analysis of Endocrine Disrupting Chemicals in Some Primary School Students in#br# Diannan Town, Dali Bai Autonomous Prefecture

Zhang Hongji, Yuan Zhiqiong

2024, 9(8):  75-79.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 013

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〕Objective: To explore the sexual maturity status of fourth to sixth grade primary school students and their knowledge,
attitudes and behaviors relevant to the endocrine disrupting chemicals （EDCs） in Diannan Town, Dali Bai Autonomous Prefecture.
Methods: Stratified random sampling method was used to select students from fourth to sixth grade in two primary schools in Diannan
Town, Dali Bai Autonomous Prefecture, and a questionnaire survey was carried out. Results: There were statistically significant
differences in the scores of EDCs-related knowledge and attitudes among students of different grades, ages and girls whether they had
secondary sexual characteristics and menarche (P<0.05). The fourth grade and sixth grade students had better EDCs-related
knowledge and attitudes than the fifth grade students. Girls without secondary sexual characteristics and menarche had better EDCsrelated
knowledge and attitudes than girls with secondary sexual characteristics and menarche. There were statistically significant
differences in the eating behavior habits of girls at the age of menarche (P<0.05). Girls who had menarche at the age of 12 and 13 had
better eating habits than girls who had menarche at the age of 11. Conclusion: Girls who had menarche at the age of 11 had poorer
dietary behavior habits; the fifth-grade students, the 11-year-old students, the female students with secondary sexual characteristics
and menarche had a poor attitude towards EDCs knowledge and more exposure to EDCs. Effective prevention and treatment programs
should be developed to cultivate students′ good daily living habits and strengthen the education of EDCs related knowledge.

Investigation on an Outbreak of Human Adenovirus Infection Caused by Swimming

Zhao Qiufang, Li Fang, Yang Rusong, Nong Luming, Ni Zhaolin, Liu Zhengnan, Wu Qiang

2024, 9(8):  80-83.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 014

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Objective: To analyze an outbreak of human adenovirus infection in a swimming club in Yuxi City, providing scientific
basis for the prevention and control of adenovirus outbreak in this area. Methods: Field epidemiological methods were used to
investigate, describe the epidemic characteristics and analyze the causes of the outbreak. Results: The epidemic lasted for 22 days, 94
cases were reported, with an incidence rate of 10.15%. The peak time of the disease was August 6 to 8, with 41 cases （accounting for
43.62%）. The clinical symptoms were mainly fever, abdominal pain, pharyngeal congestion or pharyngeal pain and conjunctival
congestion. The onset age was concentrated between 4 and 8 years old, and the male to female ratio was 0.81:1; 47 of 94 samples were
positive for adenovirus antigen, and the positive rate was 50.00%. Conclusion: This event was an outbreak of human adenovirus
infection related to swimming. The main reason for the outbreak was that the sanitation and disinfection measures in the swimming pool
were not in place.

The Status of Knowledge, Attitude and Practice about COVID-19 among Rural Doctors in Dali Bai#br# Autonomous Prefecture and Its Influencing Factors

He Zuo, Li Qingtang, Xu Ran, Chen Ling, Yang Ruixue, Li Wanying, Shi Zhaoyun

2024, 9(8):  84-89.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 015

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Objective: To understand the status of knowledge, attitude and practice （KAP） about COVID-19 among rural doctors in
Dali Bai Autonomous Prefecture （hereinafter referred to as "Dali Prefecture"）, and to explore its influencing factors. Methods: An
online questionnaire survey was conducted among 463 rural doctors in Dali Prefecture through Wenjuanxing platform. Results: The
scores of knowledge, attitude and practice and total scores about COVID-19 among rural doctors in Dali Prefecture were （7.51±1.36）,
（40.34±2.82）,（ 44.85±4.42） and（ 92.70±5.81）, respectively, with scoring rates of 75.10%, 80.66%, 89.70% and 84.25%, respectively.
Health status, education level and ethnicity were the main influencing factors of KAP of COVID-19 （P<0.05）. Attitude was positively
correlated with practice （r=0.230, P<0.001）. Conclusion: The KAP of COVID-19 among rural doctors in Dali Prefecture is at a
medium level, especially in terms of knowledge and attitude, which need to be further improved. It is necessary to strengthen the
knowledge training of prevention and control of COVID-19, and change the attitude towards prevention and control is the key to
improve the level of KAP.

（责任编辑　于　丽）#br# 张仙娟，申继传，宋立鹏，等　实习护生参与时间银行互助养老志愿服务意愿及影响因素研究#br# Research on the Willingness and Influencing Factors of Nursing Interns' Participation in Time Bank#br# Mutual Elderly-Care Volunteer Service

Zhang Xianjuan, Shen Jichuan, Song Lipeng, Zhang Mengwen, Li Mao, Zhao Guifeng

2024, 9(8):  90-95.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 016

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Objective: To investigate the willingness of nursing interns to participate in time bank mutual elderly-care volunteer
service and the influencing factors. Methods: A questionnaire survey was conducted on 409 nursing interns in a tertiary hospital in
Dali Bai Autonomous Prefecture from November 2022 to May 2023. The χ2 test was used to compare the differences in demographic
characteristics of the nursing interns, and the multifactorial Logistic regression model was used to analyze the independent influencing
factors of nursing interns′ willingness to participate in time bank mutual elderly-care volunteer service. Results: Among 409 nursing
interns, 85.1% of them were willing to participate in time bank mutual elderly-care volunteer service. Gender, education level,
participation in volunteer service, willingness to participate in elderly-care volunteer service, participation in elderly-care volunteer
service, and understanding of the time bank mutual elderly-care model were the influencing factors of the nursing interns′ willingness
to participate in time bank mutual elderly-care volunteer service （P<0.05）. Conclusion: Nursing interns have a low awareness rate of
time bank mutual elderly-care volunteer service, but a high willingness to participate.

Exploration and Practice of Intelligent Teaching Method of the Physiology Course in New Media Era

Li Juan, Qin Yan

2024, 9(8):  96-100.  DOI: 10. 3969 / j. issn. 2096-2266. 2024. 08. 017

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Physiology is one of the main courses in the medical profession, and it is a bridge course linking basic knowledge and
clinical practice, with a lot of abstract and complex teaching contents. In physiology teaching, modern science and technology are
combined with teaching strategies. Based on network platform resources and information technology, the intelligent teaching method of
physiology is explored and practiced, the whole teaching process is concerned, so as to stimulate the endogenous motivation of students,
cultivate the awareness and ability of students′ autonomous learning, and enhance their competency for future jobs.