Inhibitory Effects of Vespa velutina Extract on HepG2 Human Hepatoma Cells

doi:10. 3969 / j. issn. 2096-2266. 2021. 02. 007

Abstract

Abstract: Objective: To study the effects of Vespa velutina extract on the proliferation of HepG2 human hepatoma cells. Methods:
Vespa velutina extract of different concentrations were applied to HepG2 human hepatoma cells cultured in vitro, and MTT assay was
used to detect the absorbance value and calculate the cell inhibition rate. Results: The inhibition rates of ethyl acetate layer extract
（HJ2）, n-butanol layer extract（HJ3）, water layer extract（HJ4）and total ethanol extract（HJ5）of 1 000.0 μg/mL of Vespa velutina
on HepG2 human hepatoma cells were 60.96%, 62.95%, 62.39% and 65.98% respectively, and the inhibitory effects decreased with
the decrease of concentration; the half inhibition concentrations of HJ2, HJ3, HJ4 and HJ5 of Vespa velutina were 211.8, 173.6, 124.0
and 165.1 μg/mL, respectively. Conclusion: Vespa velutina extract has a certain inhibition effect on the growth of HepG2 human
hepatoma cells in vitro, and there is a dose-dependent effect, among which the water layer and total ethanol extract of Vespa velutina
were better.

Key words: Vespa velutina, hepatoma cells, MTT assay, inhibitory effect

CLC Number:

R735.7

Yang Qiaoling, Zhao Ruodan, Liu Xiaobo, Guo Meixian, Chen Xubing. Inhibitory Effects of Vespa velutina Extract on HepG2 Human Hepatoma Cells[J]. Journal of Dali University, 2021, 6(2): 34-38.

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