J4 ›› 2012, Vol. 11 ›› Issue (6): 23-25.

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Determination of Forsythin in Xiaoerjinqiao Granules by HPLC

Objective: To establish a method to determine the content of Forsythin in Xiaoerjinqiao Granules. Methods: High performance liquid chromatography (HPLC)was applied with the condition of 0.1% phosphoric acid-acetonitrile as mobile phase for gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 277 nm. Results: The calibration curve was linear in the range of 0.5-5.0 μg for Forsythin, r=0.999 9; the average recovery was 99.3%, and relative standard deviation RSD=0.25%(n=6).Conclusion: The established method is accurate and reliable, and can be used to determine the Forsythin in Xiaoerjinqiao Granules.   

  1. College of Pharmacy and Chemistry, Dali University, Dali 671000, China
  • Received:2011-11-18 Revised:2012-03-27 Online:2012-06-15 Published:2012-06-15

Abstract:

Objective: To establish a method to determine the content of Forsythin in Xiaoerjinqiao Granules. Methods: High performance liquid chromatography (HPLC)was applied with the condition of 0.1% phosphoric acid-acetonitrile as mobile phase for gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 277 nm. Results: The calibration curve was linear in the range of 0.5-5.0 μg for Forsythin, r=0.999 9; the average recovery was 99.3%, and relative standard deviation RSD=0.25%(n=6).Conclusion: The established method is accurate and reliable, and can be used to determine the Forsythin in Xiaoerjinqiao Granules.

Key words: Forsythin, Xiaoerjinqiao Granules, HPLC

CLC Number: