Abstract:

Objective: To study epidemiological characteristics of the β-lactamase producing Salmonella typhi in Xinyu and Shigella blaTEM-1D-type resistance gene. Methods: Isolated enzyme production strains of Salmonella typhi 6 and Shigella 13, a total of 19 strains were collected from the Xinyu Steel Central Hospital, People's Hospital of Xinyu, Chinese Medicine Hospital of Xinyu, Maternal and Child Health Hospital of Xinyu from January 2008 to June 2010. Cephalosporins post filter paper method was used to do thiophene β -lactamase qualitative detection. Affter it was defined as enzyme production strains, the lift vector plasmid was amplified by PCR blaTEM-1D resistance gene. Mark was used to measure the amplification the size of sub （bp length）; blaTEM-1D resistance gene-positive rate was kept. Results: Enzyme production strains of Salmonella typhi and Shigella blaTEM-1D resistance gene positive rates were 66.7%（4/6）,76.9%（10/13）.Conclusion: BlaTEM-1D resistance gene has made vertical transmission and morizontal transmission in the local hospitals.

Key words: Salmonella typhi, shigella, β-lactamase, blaTEM-1D resistance gene