Table of Content

15 April 2019, Volume 4 Issue 4

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Extraction Technology of the Anti-pulmonary Fibrosis Active Site from Periplaneta americana Optimized by Orthogonal Experiment

LIU Xi- , YANG Yong-Shou- , HE Zheng-Chun- , XIAO Pei-Yun

2019, 4(4):  1-6.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: Combined with the activity test in vitro, the extraction technology of the anti-pulmonary fibrosis active site
（ML-HB）from Periplaneta americana was optimized. Methods: The single factor experiment and L 9 （3 4 ）orthogonal experiment were
used to optimize extraction temperature, solvent concentration, extraction time and the number of extraction times of anti-pulmonary
fibrosis active site of Periplaneta americana, and combined with MTT method, the anti-pulmonary fibrosis activity fraction from the
different extracts was detected, the yield and IC 50 value of the active site were used as evaluation indicators to determine the optimal
extraction process of the active site of Periplaneta americana. Results: When the ratio of material to liquid was 1 ： 5（g/mL）, the
optimal extraction process of the anti-pulmonary fibrosis active site of Periplaneta americana was determined as follows: the extraction
temperature was 70 ℃, the ethanol concentration was 70%, the number of extraction times was 3, and the extraction time was 2.5 h.
Verifying the optimum conditions, the ML-HB average extraction yield was 0.59%. The IC 50 values at 24 h were 77.77, 75.17 and
76.03 μg/mL, respectively; 72.40, 71.03 and 72.26 μg/mL at 48 h, respectively; 67.24, 69.76 and 67.22 μg/mL at 72 h, respectively.
Conclusion: It stablishes the best extraction technology of ML-HB, and it provides the basis for the research and development of anti-
pulmonary fibrosis of Periplaneta americana.

Effects of Geranium strictipes on Gastroparesis Model in Mice and Gastric Smooth Muscle Strips in Rats

HU Zan, LI Cai-Gong- , LI Yin-Juan, CHEN Dun-Ya- , LA Hai, GENG Ling

2019, 4(4):  7-11.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To research the effects of Geranium strictipes on experimental gastric function in vivo and in vitro. Methods:
The gastric emptying rate（GER）, total protein content in stomach tissue and gastrin（GAS）level in serum were measured in the
atropine-induced gastroparesis model in mice. Except that the horizontal and longitudinal smooth muscle strips of fundus and body of
stomach from rats were isolated, the effects of Geranium strictipes on spontaneously contractive activities and contractive activities of
inhibited smooth muscle strips were investigated. Results: In the experiment in vivo the gastric emptying rate（GER）, total protein and
GAS contents were higher in the groups of Geranium strictipes compared with model group, and the differences were statically significant
（P < 0.01, P < 0.05）. In the experiment in vitro, the contractive frequency of the horizontal and longitudinal smooth muscle strips from
the fundus of stomach was accelerated and the contractive frequency of the horizontal and longitudinal smooth muscle strips from the
body of stomach was slowed, and the differences were statically significant （P < 0.01, P < 0.05）; moreover, the maximum tension of the
inhibited horizontal and longitudinal smooth muscle strips from the fundus and body of stomach was increased, and the difference was
statically significant（P < 0.05）. Conclusion: Geranium strictipes could enhance the GER in mice through increasing the content of
total protein in stomach tissue and the GAS in serum. Additionally, it promotes the gastric smooth muscle stripes to contract in vitro,
which may be related with the M-receptor, Ca 2+ channel and H 1 -receptor.

Screening Test on the Anti-hepatic Fibrosis Effects of Liver Capsule Combined with Drugs in Vitro

CHEN Li-Beng, YANG Jiang-Li, LI Hui, DU Yi-Min- , JIAO Su-Ru, SHAO Meng-Wan, LAI Yong

2019, 4(4):  12-17.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To study the proliferation inhibitory effects on LX-2 cells and the protective effect on L-02 cells of Ganlong
capsule combined with a variety of anti-hepatic fibrosis drugs, to evaluate the anti-hepatic fibrosis effect of Ganlong capsule combined
withvalsartan,Xiaochaihu,methylprednisolone,breviscapine,colchicine,polyenephosphatidylcholine,silybin,compound glycyrrhizin in
vitro. Methods: Ganlong capsule alone and combination with different dosages of anti-hepatic fibrosis drugs were dealt with LX-2
cells and L-02 cells by using two factors and five levels experiment. The survival rate of L-02 cells and the inhibition effect of LX-2
cells were tested by MTT method. Results: When the fa was less than 0.8 and the CI value was less than 1, Ganlong capsule combined
with silybin and compound glycyrrhizin showed synergistic effects on the proliferation inhibition of LX-2 cells. 0.496 mg/mL Ganlong
capsule combined with 1.563 mg/mL compound glycyrrhizin and 0.438 mg/mL silybin showed the most obvious proliferation inhibitiory
value on LX-2 cells, and L-02 cells had the best activity rate. Conclusion: Ganlong capsule could enhance the anti-hepatic fibrosis
effect of silybin and compound glycyrrhizin in vitro.

Effects of Ethanol Extracts from Different Parts of Taraxacum mongolicum Whole Herb on Cariogenic Bacteria

YANG Qian, SHU Sai-Ying, DAN Chao-Fang, DAN Cheng-Yu, LA Hai

2019, 4(4):  18-21.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective：The effects of ethanol extracts from different parts of Taraxacum mongolicum on the growth and acid
production of two main kinds of cariogenic bacteria are preliminarily investigated in vitro. Methods：The minimum inhibitory
concentration (MIC) and minimum bactericidal concentration (MBC) of ethanol extracts from root, stem, leaf, flower and whole herb of
Taraxacum mongolicum against Streptococcus mutans and Actinomyces viscosus were determined by double dilution method，and the
changes of acid production of the two main kinds of bacteria were observed at 4 concentration gradients lower than MIC. Results: The
ethanol extracts from different parts of Taraxacum mongolicum had different inhibitory effects on Streptococcus mutans and Actinomyces
viscous, and the Taraxacum mongolicum stem had the strongest inhibition ability against Streptococcus mutans. The Taraxacum
mongolicum flower and whole herb had the best inhibitory effect on Actinomyces viscous. Ethanol extracts from different parts of
Taraxacum mongolicum could inhibit the acid production of the two main kinds of bacteria, but weaker than chlorhexidine acetate.
Conclusion：The ethanol extracts of Taraxacum mongolicum root, stem, leaf, flower and whole herb have different inhibitory effects on
the growth and acid production of two main kinds of cariogenic bacteria, and are sensitive to Actinomyces viscosus.

Effect of the Extract of Taraxacum mongolicum on Remineralization of Bovine Incisor Root Caries

DAN Cheng-Yu, DAN Chao-Fang, YANG Qian, SHU Sai-Ying, LA Hai

2019, 4(4):  22-25.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: This study examines whether the extract of Taraxacum mongolicum can early prevent dental caries by
intervening the remineralization process, by building early dental caries in vitro and simulating demineralization and remineralization
process on the root surface. Methods: 32 bovine incisors with artificial caries were randomly divided into 4 groups: blank control
group, demineralization group, Taraxacum mongolicum extract group, and NaF group. Bovine incisors were treated with extracorporeal
pH cycle, and caries were observed by polarized light microscope（PLM）and confocal laser scanning microscope（CLSM）. Results:
The differences of total and average fluorescence amount between Taraxacum mongolicum extract group and blank control group were
statistically significant（P<0.05）. The differences of total and average fluorescence amount between Taraxacum mongolicum extract
group and NaF group were not statistically significant（P>0.05）. Conclusion: Taraxacum mongolicum extract can prevent early caries
by remineralization.

Study on the Application of PFGE in Mtb Genotyping of HIV/Mtb Co-infection

HAO Xian-Bin, NIE Heng, WANG Xu-Dong, TUN Li-Xian

2019, 4(4):  26-30.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To preliminarily investigate the application of PFGE in Mtb genotyping of HIV/Mtb co-infection. Methods: 27
Mtb strains of HIV/Mtb co-infection were collected from Dali area. Pulsed field gel electrophoresis（PFGE）was used to identificate
the genotyping of Mtb, then Quantity One and BioNumerics 6.6 software were used for digital processing and clustering analysis for the
experimental results. Results: The 27 strains could be categorized into 5 gene clusters. However, the cluster A was the predominant
prevalent strain and the H37Rv was also in cluster A, Simpson's index of diversity（D）= 0.698. Conclusion: PFGE typing method is
suitable for genotyping of Mtb in HIV/Mtb co-infection, and it is proved to be one of the best ways for the identification.

Effects of Periplanteta americana Peptide on Lymphocytes in Peripheral Blood of MFC Tumor Bearing Mice

JIANG Lun- , ZHENG Xiao-Chun- , CHEN Ze-Feng- , ZHANG Cheng-Gui- , LV Yan, BAI Li

2019, 4(4):  31-35.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: In order to study the influence of Periplanteta americana peptide on lymphocytes in the peripheral blood of
MFC tumor bearing mice and explore its anti-tumor mechanism. Methods: Mouse gastric cancer cells（MFC）were cultured in vitro.
We established a subcutaneous tumor model in BALB/c mice with MFC cells，and divided them into 5 groups including model group，
CTX group，the Periplanteta americana peptide extract CⅡ-3 high and low dose groups，synthetic peptide HFDT1 group, and normal
BALB/c mice were used as normal control group. The model group and the normal group were given normal saline by gavage, while
other groups were administrated with corresponding drugs for 10 days. The changes of lymphocytes in the peripheral blood were
analyzed by automatic blood cell counter and flow cytometry after administration. Results: CⅡ-3 and short peptide HFDT1 increased
the number of lymphocytes，and the ratios of B cell，T cell and its subsets, meanwhile they did not decrease the content of NK cell in
the peripheral blood. Conclusion: Periplaneta americana extract CⅡ-3 and synthetic peptide HFDT1 enhanced the anti-tumor
effects of specific immunity and non-specific immunity in tumor bearing mice.

Analysis on the Tissue Expression Profile of Mterf1 Gene in Kunming Mice

WANG Wei-Shi, ZI Jia-Ji- , YANG Yong-Qin- , YAN Chang-Bao, DAI Chi-Ping- , TU Min, XIONG Wei

2019, 4(4):  36-40.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To analyze the tissue expression profile of mouse mitochondrial transcription termination factor 1（Mterf1）
gene in 10 different tissues of Kunming mice. Methods: Real-time RT-PCR was performed to study the mRNA expression level of
Mterf1 gene in different tissues of mice, and Western Blot was preformed to study the protein expression level of Mterf1 protein in
different tissues of mice. Results: Compared with the rest of the tissues, the expression level of Mterf1 gene was highest in the brain,
heart, skeletal muscle and bladder, secondly in the liver, kidney and spleen, but relatively lower in lung, pancreas and ovary.
Conclusion: Th

Preliminary Study on Employment-oriented Teaching Reform of Pathology

LI Shui-Xian, DIAO Shan-Yu, YANG Wen-Juan-

2019, 4(4):  41-44.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective：To explore the effect of employment-oriented teaching system optimization reform in pathology teaching.
Methods: The students of grade 2015 and grade 2016 majoring in clinical medicine at Dali University were selected as the research
subjects. The traditional teaching method was adopted in grade 2015, while the employment-oriented optimized teaching system was
adopted in grade 2016. The difference of learning effect between the two groups was analyzed and compared through the final
examination. Results: The passing rate, good rate and excellent rate of the students in grade 2016 were higher than those of the grade
2015, and the differences were statistically significant （P < 0.05）. Conclusion: Compared with the traditional teaching, the
employment-oriented optimization teaching reform is conducive to improve the teaching quality of pathology and the learning effect of
students.

Clinical Observation of Diammonium Glycyrrhizinate Enteric-coated Capsule in Preventing Liver Injury Induced by Anti-tuberculosis Drugs

MO Rong, LIU Sha, FAN Zhi-Jian, LI Meng-Wu

2019, 4(4):  45-47.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To observe the clinical efficacy of diammonium glycyrrhizinate enteric-coated capsule on preventive liver
protection during anti-tuberculosis treatment. Methods: A total of 127 patients with newly diagnosed smear-positive pulmonary
tuberculosis admitted to the Sixth Affiliated Hospital of Dali University from March 2013 to December 2014 were enrolled. All patients
received standardized anti-tuberculosis treatment and were divided into observation group and control group according to the principle
of random number table. The patients in the observation group were given liver protection treatment with diammonium glycyrrhizinate
enteric-coated capsule during anti-tuberculosis treatment，while the control group did not receive liver protection treatment during the
treatment period. The incidence of liver damage, the time of occurrence, and the extent of liver damage were compared between the two
groups. Results: In the observation group, 11 cases had liver damage, the incidence rate was 18.0%; in the control group，16 cases had
liver damage, the incidence rate was 26.7%. There was no significant difference in the incidence of liver damage between the two
groups (P>0.05). The average time of liver damage in the observation group occurred after 2 months of treatment, while that of the
control group was half a month later, and the time of liver damage occurred in the two groups was statistically different (P<0.05).
Compared the degree of liver damage between the two groups, the ALT, AST, and TbiL indexes of the control group were all higher
than the observation group, and there was no statistical difference between the two groups(P>0.05). Conclusion: Diammonium
glycyrrhizinate enteric-coated capsule can effectively prevent the occurrence of drug-induced liver damage during anti-tuberculosis
treatment, but for patients without basic liver disease, and people without susceptibili

Analysis of Nutritional Intervention on 119 Patients Suffering from Pulmonary Tuberculosis
Complicated with Intestinal Tuberculosis

SHU Hui-Qiong, HUANG Gong-Li, MO Rong, LI Meng-Wu

2019, 4(4):  48-51.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To study the nutritional status and the effectiveness of nutritional intervention of the patients suffering from
pulmonary tuberculosis (PTB) complicated with intestinal tuberculosis. Methods: 119 PTB complicated with intestinal tuberculosis
patients treated in the Sixth Affiliated Hospital of Dali University were chosen as the observation group, and 213 PTB patients
hospitalized in the same hospital were chosen as the control group. Nutritional risk screening was conducted and side effects caused by
anti-tuberculosis drugs were recorded. Results: The nutritional status indicators of the observation group were lower than those of the
control group and the nutritional risk of both groups showed improvement after nutritional intervention and anti-tuberculosis treatment；
the control group was significantly better than that of the observation group, with significant difference (P<0.01). Length of treatment
time of the control group was significantly shorter than that of the observation group, with significant difference (P<0.01). There was
significant difference in incidence of gastrointestinal reactions and liver damage caused by anti- tuberculosis drugs between the
observation group and the control group (P<0.05). Conclusion: In addition to receiving anti-tuberculosis therapy, patients suffering
from PTB complicated with intestinal tuberculosis should rest well and maintain good nutrition to support completion of treatment,
reduce complications, shorten hospital stays, and reduce economic burden.

Comparative Study on the Clinical Effects of Laparoscope-assisted and Abdominal Radical Gastrectomy for Gastric Cancer

LAI Jing-Ye, CHEN Hui-Yong, DIAO Feng

2019, 4(4):  52-55.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To compare the clinical effects and postoperative inflammatory responses of laparoscope - assisted and
abdominal radical gastrectomy for gastric cancer. Methods: The clinical data of 156 patients diagnosed with gastric cancer and
performed with radical gastrectomy in the Third Affiliated Hospital of Dali University from July 1, 2014 to March 31, 2018 were
retrospectively analyzed, including 81 cases of laparoscope-assisted radical gastrectomy and 75 cases of abdominal radical
gastrectomy. Results: The amount of intraoperative blood loss, postoperative first ventilation time and hospital stay of patients in the
laparoscope-assisted group were significantly better than those in the abdominal group, and the difference was statistically significant
（P<0.001）. The operative time of the abdominal group was significantly shorter than that of the laparoscope-assisted group, and the
difference was statistically significant （P<0.05）. The difference of the number of lymph nodes dissected between the two groups wasn't
statistically significant（P＞0.05）. Conclusion: The laparoscope-assisted radical gastrectomy has less bleeding, faster recovery and
less postoperative inflammatory reaction than the abdominal radical gastrectomy.

Meta Analysis of Pancreatic Fistula after Pancreaticoduodenectomy with Different Pancreaticojejunostomy

ZHANG Wen-Jie, TAN Yun-Bei-

2019, 4(4):  56-63.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective：To systematically evaluate the effect of duct- to- mucosa and invagination pancreaticojejunostomy on
pancreatic fistula after pancreatoduodenectomy. Methods：The relevant literature was searched in Chinese and foreign biomedical
databases. The quality assessment of the literature was based on the Jadad quality scoring system. Meta analysis was performed using
RevMan 5.3 software. Results：A total of 11 articles were included . There were 591 cases of duct-to-mucosa group and 607 cases of
invagination group. Meta analysis showed: incidence of total pancreatic fistula［RR=0.83, 95% CI: 0.65-1.06, P=0.14］, incidence of
pancreatic fistula A［RR=1.40, 95% CI：0.90-2.19, P=0.13］, incidence of pancreatic fistula B［RR=0.65, 95% CI: 0.19-2.21, P =
0.49］, incidence of pancreatic fistula C［RR=0.95, 95% CI: 0.40-2.30, P=0.92］, incidence of soft and hard pancreas pancreatic
fistula［RR = 1.06, 95% CI: 0.79-1.43, P = 0.68］,［RR=1.14, 95% CI: 0.80-1.62, P = 0.47］were not statistically significant.
Conclusion：There is no significant difference in the incidence of pancreatic fistula after pancreatoduodenectomy between two kinds
of pancreaticojejunostomy.

Comparison of Efficacy of Two Types of Transobturator Tension-free Tape in the Treatment of Moderate
and Severe Female Stress Urinary Incontinence

WANG Ling, GAO Jian-Yong, LEI Yi, MU Jian-Jun, BAI Bing, JIAN Ji-Qiong, DAO Gong-

2019, 4(4):  64-67.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To assess the safety and efficacy of "inside-out"（TVT-O）and "outside-in"（TOT）transobturator tension-
free tape for surgical of moderate and severe female stress urinary incontinence（SUI）. Methods: The clinical data of 122 patients
with moderate and severe SUI were retrospectively analyzed, including 80 cases of TVT-O and 42 cases of TOT who were followed up
for 12 months. The operation time, the amount of bleeding, the complications and the follow-up were recorded. Results: In groups of
TVT-O and TOT, there were 6 cases and 4 cases with transient urinary retention respectively, which extended the time of indwelling
urethral catheter. At the same time, there were 3 cases and 2 cases of pain in the medial thigh and groin area, and then self remission.
The differences were not statistically significant between the two groups in the operation time, the amount of bleeding and postoperative
complications（P>0.05）. There were no bladder injury, permanent retention of urine, and the sling exposure in the two groups.
However, there were 3 cases of vaginal injury and injury to the index finger in TOT group. Conclusions: TVT-O and TOT are
minimally invasive, safe and effective methods for the treatment of moderate and severe female SUI. However, the TOT is prone to
vaginal injuries and injuries to the doctors when the surgical puncture is punctured. Therefore, TVT-O is more suitable for clinical
practice.

Effects of Overnight Incubation Culture on the Treatment of Different Quality Frozen-thawed Embryo

TUN Rui, ZHANG Li-Rong, HU Zhi-Fu, XU Cheng-He- , ZHANG Re-Feng- , LUO Da-Ji

2019, 4(4):  68-70.  DOI: R321.1

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Objective: To explore the effect of overnight incubation culture on the treament of different quality frozen-thawed-embryo
and blastocyst. Methods: We retrospectively analyzed the patient data of 1 326 FET cycles conducted in the First Affiliated Hospital
of Dali University. They were divided into the group of overnight incubation culture and the group of conventional culture according to
the purpose of the study. We compared the difference of the clinic pregnancy rates of the patients respectively transferred good-quality
embryo, non-good-quality embryo, good-quality blastocyst and non-good-quality blastocyst between two groups. Results: There were
no differences of the clinic pregnancy rates of patients transferred the good-quality embryo or non-good-quality blastocyst between two
groups. However, the clinic pregnancy rate of patients transferred the non-good-quality embryo in the group of overnight incubation
culture was significantly higher than those in the group of conventional culture（36.36% vs. 25.89%, P=0.021）, while the clinic
pregnancy rate of patients transferred the good-quality blastocyst in the group of overnight incubation culture was significantly lower
than those in the group of conventional culture（51.25% vs. 65.13%, P=0.040）. Conclusion: Overnight incubation culture is beneficial
to improve the treat

Correlation Analysis on Insulin-like Growth Factor-1 and the Pathogenesis of Polycystic Ovary Syndrome

LI Hai-Yan- , CHANG Qi

2019, 4(4):  71-74.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: The relationship between IGF- 1 and the pathogenesis of PCOS was summarized systematically, which
provided more references for the study of the pathogenesis and endocrine disorder of PCOS. Methods: From the aspects of structure,
function, biological function and endocrine mechanism of IGF-1 in PCOS, this paper retrieved and sorted out the recent literature on
endocrine disorders caused by IGF-1 in the pathogenesis of PCOS, and summarized and analyzed the related literature. Results: IGF
is a kind of multifunctional proliferating factor, which is mainly regulated by IGFBP and IGF- 1R. It can promote cell growth,
proliferation and differentiation, regulate metabolism, angiogenesis, and play important biological roles in all aspects of reproductive
process. Conclusion: The study on the pathogenesis and endocrine of PCOS by IGF- 1 is not only helpful to understand the
physiological fu

Analysis of Risk Factors for Knee Osteoarthritis in Dali Area of Yunnan

DIAO Chang, LI Gong, HU Zhi-Fu, SUN Zhe, SUN Chao-Jun

2019, 4(4):  75-78.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To study the risk factors of knee osteoarthritis（KOA）in Dali area and provide evidence for prevention and
treatment of KOA in Dali area. Methods: KOA screening and epidemiological survey were conducted among 315 permanent residents
in Dali area who saw doctors in the First Affiliated Hospital of Dali University. Case control study and unconditional logistic regression
analysis were conducted to study the risk factors of KOA. Results: Logistic regression analysis showed that sex（OR=1.827, sig=
0.205）, age（OR=1.252, sig=0.000）, BMI（OR=1.298, sig=0.000）, family history（OR=12.619, sig=0.002）, toilet habits（OR=0.000,
sig=0.999）, living environment（OR=25.857, sig=0.015）, triglyceride levels（OR=12.703, sig=0.000）, smoking（OR=0.492, sig=
0.466）, drinking（OR=3.216, sig=0.235）, type of work（OR=2.949E8, sig=0.999）. Conclusion: Age, BMI, family history, triglyceride
levels, and living environment are the risk factors of KOA in Dali area.

Clinical Distribution and Resistance Analysis of Haemophilus Strains Isolated from Pediatric Patients with Respiratory Tract Infection in Dali Region

XIE Ge-Xin, YAN Ping, LEI Ying, DIAO Wei-Dong-

2019, 4(4):  79-82.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To explore the clinical distribution and drug resistance of Haemophilus strains isolated from children with
respiratory tract infection in the First Affiliated Hospital of Dali University, and provide evidence for antimicrobial therapy. Methods:
The samples from the respiratory tract of children in the First Affiliated Hospital of Dali University from April 2013 to April 2018 were
collected, the application of automatic microorganism identification and drug sensitive analysis system was used to identify the species,
the K-B method was adopted for drug susceptibility analysis. Results: A total of 114 Haemophilus strains were isolated. Among them,
H.influenzae and H. parainfluzae accounted for 50.00% and 44.74%, respectively. The sensitivity rate to meropenem of these strains
was greater than 90%. The resistance rates to ampicillin of these strains was greater than 50%. Conclusion: H.influenzae and H.
parainfluzae have lower susceptibility rates to compound sulfamethoxazole and ampicillin, respectively. However, these two strains
have higher sensitivity to meropenem. Clinicians should use antibiotics reasonably and appropriately in order to decrease the
generation of drug-resistant strains.

CT Diagnostic Method and a Literature Review of Pneumocystis Pneumonia Secondary to AIDS

LI Yun, SHI Hui-Xian

2019, 4(4):  83-86.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To investigate the imaging diagnosis of pneumocystis pneumonia（PCP）in AIDS. Methods: PCP in Shanghai
Public Health Clinical Center of AIDS from 2017 to 2018 were analyzed. Results: The patients with double lung saw glass shadow or
fine reticular nodule shadow, with the distribution of the double lung near the door and the middle and lower lung, showing a map-
shaped distribution. Conclusion: PCP is common in patients with AIDS and congenital immunodeficiency. The treatment absorption is
slow, the clinical symptoms are obvious, the lung signs are mild, and the anti-infection, anti-fungal are ineffective. In the imaging of
AIDS patients, if symmetrical glass shadow is found, midlung is dominant and map- shaped images are shown, PCP should be
considered first.

Comparative Analysis of HIV/AIDS Infection between Intravenous and Non-intravenous Drug Users

LI Hong-Liu, ZHANG Jian-Bei, SHEN Yuan-Yang

2019, 4(4):  87-89.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To compare the baseline and clinical indicators of HIV/AIDS patients infected by intravenous and non-
intravenous drug users in Dali from 2005 to the first half of 2018.To clarify the current situation of intravenous drug users and provide
theoretical basis for health decision-making and preventive measures. Methods: HIV/AIDS patients were classified according to
different infection routes, baseline and clinical data were collected and analyzed. Results: Most of the patients infected with HIV/AIDS
by intravenous drug use in Dali City were males, with an average age of（44.95±5.93）years. The baseline CD4
+ T cell value was lower
than that of other transmission routes, and the concurrent prevalence of hepatitis C was highest with 78.15%. There were significant
differences in mortality and adherence rate between intravenous and non-intravenous drug users（P<0.001）, and adherence rate was
lower than that of non-intravenous drug users, and the mortality rate was higher than that of non-intravenous drug users. Conclusion:
To strengthen the screening of middle-aged male drug users, early detection, early diagnosis and early treatment can reduce the
infection and death of patients.

Analysis of the Content of Metal Elements in Coptis chinensis and Its Planting Soil in Shizhu County of Chongqing

QI Miao, HE Min, LU Feng, LI Dui-Xian, DIAO Hui, SUN Ji-Yang, CHOU Rong-Xiang

2019, 4(4):  90-93.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To understand the content of metal elements in different species (planted and wild) of Coptis chinensis with
different growth years and the planting soil in Shizhu County of Chongqing. Methods: The flame atomic absorption spectrometry
(FAAS) was adopted to determine the contents of eight metal elements Fe, Mn, Zn, Pb, Ca, Cu, Cr, Cd in samples. Results: The content
of metal elements in Coptis chinensis was Ca>Mn>Fe>Zn>Cu>Pb>Cd>Cr. Comparison between the content of metal elements in Coptis
chinensis and the heavy metal limit index of Chinese medicinal materials in the national standard showed that the differences of Pb and
Cu were statistically significant (P<0.05). Compared the content of metal elements in Coptis chinensis and the flower of Coptis chinensis,
the differences of Fe, Mn, Zn, Ca were statistically significant (P<0.05), the content of Mn and Zn was higher in Coptis chinensic, and
the content of Fe and Ca was higher in the flower of Coptis chinensis; compared the content of metal elements in Coptis chinensis and
planting soil, the differences of Fe, Zn, Pb, Ca, Cu were statistically significant (P<0.05), the content of Zn, Ca, and Cu was higher in
Coptis chinensis, and the content of Fe and Pb was higher in the planting soil; compared the content of metal elements in planted and
wild Coptis chinensis, the differences were not statistically significant (P＞0.05). Conclusion: The content of metal elements in Coptis
chinensis and the flower of Coptis chinensis，planted and wild Coptis chinensis are consistent. The content of metal elements in the
planting soil, Coptis chinensis and the flower of Coptis chinensis are affected by geological factors.

Investigation and Countermeasure of Occupational Exposure Knowledge of Nursing Students in Infectious Disease Hospital

SUN Ya-Ping, CENG Qi, ZHANG Min, WU Hun-Li

2019, 4(4):  94-97.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To understand the knowledge level of nursing students, including knowledge of infectious diseases and
occupational exposure protection. To standardize the management of occupational protection for nursing students and provide strategies
for clinical practice teaching in infectious disease hospitals. Methods: From July 2017 to March 2018, three questionnaire surveys
were conducted among 100 nursing students in respiratory department of a "Three A" infectious disease hospital before pre-job
training, 4 months after internship and 8 months after internship. Results: Before pre-job training, the awareness rate of occupational
exposure-related knowledge was low. After four months of internship, the awareness rate increased significantly; after eight months, the
awareness rate was more than or equal to 98%. Conclusion: During pre-job training and internship, it is very important to strengthen
the education of nursing students' occupational protection knowledge and improve their occupational protection and cognitive ability,
which can reduce the probability of nursing students' occupational exposure.

Effect of Dynamic Telephone Follow-up on Quality of Life and Pulmonary Function in COPD Patients

DENG Xiao-Li, LI Yue-Lu

2019, 4(4):  98-100.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To explore the effect of dynamic telephone follow-up in the follow-up of COPD patients. Methods: 122 COPD
patients admitted in the First People's Hospital of Yunnan Province from June 2017 to June 2018 were selected as the study subjects,
and divided into observation group and control group. The control group was followed up by routine telephone calls, while the
observation group was followed up by dynamic telephone calls. The intervention effect was evaluated by pulmonary function and CAT
scale. Results: After 6 months of intervention, the PEFR and FEV 1 in the observation group were higher than those in the control group
（P<0.05）, and the CAT score in the observation group was lower than that in the control group（P<0.01）. Conclusion: Dynamic
telephone follow-up can improve pulmonary function and quality of life of COPD patients after discharge.