西南石油大学学报(自然科学版) ›› 2017, Vol. 39 ›› Issue (4): 176-182.DOI: 10.11885/j.issn.16745086.2016.06.30.03

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The Selection of Paraffin-degrading Bacteria and Their Effects upon the Rheological Characteristics of Heavy Crude Oil

WANG Weiqiang, LI Jia, WANG Guofu, ZHANG Xiaobo   

  1. College of Petroleum Engineering, Liaoning Shihua University, Fushun, Liaoning 113001, China
  • Received:2016-06-30 Revised:2017-06-23 Online:2017-08-01 Published:2017-08-01

Abstract: The extraction of heavy crude oil presents challenges due to the high viscosity and immobility of the emulsions. Microbes may offer a more economical and environmentally friendly alternative to conventional methods for improving the rheological characteristics of heavy crude oil. A strain of paraffin-degrading bacteria (ZL-7) was screened from activated sludge collected from the Liaohe Oilfield. It was found that the optimal cultivation conditions occurred during a culture period of seven days at 45 ℃. The treatment of heavy crude oil using bacteria-containing enriched culture media (i.e., a bacterial suspension) for seven days resulted in a pronounced reduction of viscosity and paraffin content. Focused beam reflectance measurements were used to monitor the chord length distribution and droplet diameter distribution of the heavy crude oil during treatment. After treatment, it was found that the percentage of small and large diameter droplets had increased and decreased, respectively. This shows that the average diameter of the crude oil droplets decreased after treatment, which is beneficial to the preparation and isolation of heavy crude oil emulsions.

Key words: paraffin-degrading bacteria, heavy crude oil, viscosity and paraffin content reduction, droplet diameter

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