大理大学学报 ›› 2022, Vol. 7 ›› Issue (2): 22-28.

• 药学 • 上一篇    下一篇

藏区濒危药用植物独一味的叶绿体基因组特征

蒲 芬1,张德全12 *   

  1. 1.大理大学药学院,云南大理 6710002.云南省滇西抗病原植物资源筛选研究重点实验室(培育),云南大理 671000

  • 收稿日期:2021-06-25 修回日期:2021-09-15 出版日期:2022-02-15 发布日期:2022-03-28
  • 通讯作者: 张德全,教授,博士,E-mail: zhangdeq2008@126.com。
  • 作者简介:蒲芬,硕士研究生,主要从事药用植物基因组学研究。
  • 基金资助:
     国家自然科学基金项目(32060091);大理大学中药资源与民族药创新团队项目(ZKLX2019318

Chloroplast Genome Characteristics of a Tibetan Endangered Medicinal Plant Lamiophlomis rotata

Pu Fen1 Zhang Dequan12*   

  1. 1.College of Pharmacy Dali University Dali Yunnan 671000 China 2. Yunnan Key Laboratory of Screening and Research

    on Anti-Pathogenic Plant Resources from Western YunnanCultivation), Dali Yunnan 671000 China

  • Received:2021-06-25 Revised:2021-09-15 Online:2022-02-15 Published:2022-03-28

摘要:

目的:解析藏区药用植物独一味的叶绿体全基因组特征及确定其系统发育位置。方法:利用二代测序技术对独一味进行测序,经组装及注释,得到叶绿体全基因组序列;对其基因组特征进行解析,并结合唇形科近缘类群的叶绿体基因组序列,进行系统发育分析。结果:独一味叶绿体全基因组为典型的四分体结构,全长151 808 bp,共注释了107个基因。基于贝叶斯法(BI)和最大似然法(ML)构建系统树的拓扑结构基本一致,独一味属与水苏属聚为一支。结论:独一味叶绿体全基因组特征首次得到解析,系统发育研究支持独一味属与水苏属近缘的传统分类处理,为独一味及其相关种的物种鉴定、资源保护及系统进化等研究奠定了基础。

关键词: font-family:宋体, ">独一味;叶绿体基因组;系统发育;二代测序;药用植物

Abstract:

Objective To reveal the complete chloroplast genome characteristics of a medicinal plant in Tibetan region namely Lamiophlomis rotate and explore its phylogenetic position. Methods In this study the Next-Generation Sequencing technology was adopted to perform DNA sequencing for this species and its complete chloroplast genome was obtained after assembly and annotation. Meanwhile the genome characteristics were revealed and its phylogeny was systematically analyzed combined with the published chloroplast genomes of related species in Lamiaceae. Results The complete chloroplast genome of L. rotata was a typical quadripartite structure the total length of which was totally 151 808 bp and 107 genes were annotated. The topological structures between the BayesianBI and the maximum likelihoodML were extremely similar proving that Lamiophlomis and Stachys were gathered into one clade. Conclusion The complete chloroplast genome characteristics of L. rotata were revealed for the first time and its phylogenetic study supported the traditional treatment of regarding Lamiophlomis and Stachys as closely related genera. This study could provide scientific basis for studies such as species identification resource conservation and phyletic evolution of L. rotata and related species.

Key words: font-family:SimSun, ">Lamiophlomis rotatafont-family:SimSun, ">;font-family:SimSun, "> chloroplast genomefont-family:SimSun, ">;font-family:SimSun, "> phylogenyfont-family:SimSun, ">;font-family:SimSun, "> Next-Generation Sequencingfont-family:SimSun, ">;font-family:SimSun, "> medicinal plant

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