Abstract:

Objective: To study the anti-hepatic fibrosis effects of extracts from walnut shell of Juglans sigillata D. in vitro（HTK-D, HTK-F, HTK-DEF）. Methods: The MTT assay was used to observe the effects of the extracts from walnut shell of Juglans sigillata D. on hepatic stellate cells'（HSC）propagation and their cytotoxicity on normal human liver cell（L-02）. ELISA method was used to detect the contents of TGF-β1, collagen Ⅰ and collagen Ⅲ in the supernatant of HSC. Results: Both HTK-D and HTK -DEF（the concentration from 15.63 μg/mL to 1 000.00 μg/mL）had inhibiting effects on HSC after 48 hours and 72 hours（P＜0.05）. HTK-F（62.50 μg/mL-1 000.00 μg/mL）could inhibit the proliferation of HSC for 48,72 h（P＜0.05）. The values of CC50 were >1 000.00 μg/mL, 354.73 μg/mL, 457.09 μg/mL accordingly after L-02 was cultured with HTK-D, HTK-F and HTK-DEF respectively for 48 h. HTK-D, HTK-F and HTK-DEF could reduce the production of TGF-β1 in supernatant for 48 h at concentration between 62.50 μg/mL and 1 000.00 μg/mL in a concentration dependent manner（P＜0.05）. HTK-D, HTK-F and HTK-DEF could decrease the production of collagen Ⅰand collagen Ⅲ in supernatant at different concentration gradient（P＜0.05）. Conclusion: Extracts from walnut shell of Juglans sigillata D. in vitro have definite effects on anti- hepatic fibrosis． The mechanism may be related to inhibiting HSC propagation and reducing the production of TGF-β1, collagen Ⅰ and collagen Ⅲ.

Key words: walnut shell of Juglans sigillata D., extracts , anti-hepatic fibrosis, transforming growth factor-β1, collagen type Ⅰ, collage type Ⅲ