Journal of Dali University ›› 2020, Vol. 5 ›› Issue (2): 61-64.DOI: 10. 3969 / j. issn. 2096-2266. 2020. 02. 013
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Wu Chunhua 1 , Li Xingyun 2 , Zhang Yue 2 , Yang Daixian 2 , Liu Dongyan 2 , Du Wei 1*
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Abstract: Objective: To study the effect of PPAR-alpha agonist WY14643 on the expression of MMP-9 in hepatocellular carcinoma. Methods: Twenty-four rabbits with VX2 hepatocellular carcinoma were established and randomly divided into four groups: the control group, the low, the medium and the high dose group, each group consisting of 6 rabbits. Each medicated group was given WY14643 at low(1 mg/kg), medium(3 mg/kg)and high(6 mg/kg)doses via ear vein respectively. The control group was given the same amount of 0.9% sodium chloride solution(NS). The levels of MMP-9 in serum of each medicated group were determined before and 12, 24 and 48 hours after administration. The control group was detected by blood sampling at the same time, and the SSPS 22.0 was used for statistical analyses. Then the experimental animals were killed and the samples of tumor tissues and adjacent liver tissues were obtained for histopathological examination. Results: Compared with the control group, the serum levels of MMP-9 in WY14643 medium and high dose groups were significantly lower(P < 0.05). The tumor cells in the four groups showed obvious pathological changes; in the control group and the low dose group, a large number of steatosis and apoptotic cells were observed in the liver tissues adjacent to cancer, and a large number of inflammatory cells infiltrated in the surrounding tissues. Only mild steatosis and a small number of inflammatory cells infiltrated in the liver tissues adjacent to cancer in middle and high dose groups. Conclusion: WY14643 has a certain inhibitory effect on MMP-9 of VX2 hepatocellular carcinoma in rabbits.
Key words: WY14643, matrix metalloproteinase-9, rabbit VX2 hepatocellular carcinoma
Wu Chunhua,Li Xingyun,Zhang Yue,Yang Daixian,Liu Dongyan,Du Wei . Effects of PPAR-alpha Agonist WY14643 on the Expression of MMP-9 in Rabbit VX2 Hepatocellular#br# Carcinoma[J]. Journal of Dali University, 2020, 5(2): 61-64.
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URL: http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/10. 3969 / j. issn. 2096-2266. 2020. 02. 013
http://journal15.magtechjournal.com/Jwk_dlxyzk/EN/Y2020/V5/I2/61