Table of Content

15 December 2018, Volume 3 Issue 12

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On Entire Solutions of Fermat Type Functional Equations

DUAN Jiang-Mei, HAN Yan, YANG Hui-Juan

2018, 3(12):  1-3.  DOI: 10. 3969 / j. issn. 2096-2266.

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This paper investigates the existence of entire solutions of the functional equationf ¹²₁ (z)+ f ¹²₂ (z)+ f ¹²₃ (z)+ f ¹²₄ (z)= 1 and new results are found.

Examples on the Methods for Finding the Ranges of Functions

HE Zhi-Xiong

2018, 3(12):  4-7.  DOI: 10. 3969 / j. issn. 2096-2266.

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The solution of function ranges is a comprehensive problem, which can involve multiple thinking methods and knowledge
points. This paper intends to select a typical range problem, to examine the problem from different angles through multiple solutions, to
find the distinctive entry points for solving the problem, to seek the connection between the current information and the existing basic
knowledge, and to train students to use mathematics comprehensively in problem-solutions. In this way, students can form a more
comprehensive and complete cognitive structure, and have a deep and flexible understanding of the knowledge structure.

Design of Insect Intelligent Habitat Monitoring System in Erhai Wetland

LUO Gui-Lan, ZHANG Mei, HUANG Wei, ZHANG Li-Na

2018, 3(12):  8-13.  DOI: 10. 3969 / j. issn. 2096-2266.

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In order to solve the problem of real-time collection and intelligent monitoring of insect habitat data in Erhai wetland, an
insect habitat mobile monitoring system was designed based on Internet of Things and Android system. A variety of insect habitat
factors such as temperature, humidity, light and pH value of soil were collected in real time through the sensor module of the Internet of
Things. With the help of cloud service, the functions of online transmission, storage, processing and display of habitat data were
realized in the system. Experimental application tests showed that: the system can monitor the update state of the insect habitat data
table in the backend cloud database in real time through the mobile terminal. It could meet the requirements of intelligent monitoring
performance and provide a modern information means for the study of insect population and ecological protection.

Complex Network Community Structure Extraction Based on Continuous Hopfield Network

DAI Ting-Ting, DONG Yan-Shou, HAN Yan

2018, 3(12):  14-17.  DOI: 10. 3969 / j. issn. 2096-2266.

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As a new subject, complex networks has attracted the attention of researchers from different disciplines. Community
structure is one of the key structures of complex networks, so it is a very important task to analyze the community structure of complex
networks accurately. This paper studies the problem of extracting the community structure of complex networks, and proposes a
continuous neural network（CNN）algorithm. It is proved that for any given initial value, the continuous neural network algorithm
converges to the eigenvector of the maximum eigenvalue of the network modularity matrix B. Therefore, according to the stability of the
evolution of the network symbol, two community structures will be obtained.

Research on Handwritten Hmong Character Recognition Based on Probabilistic Neural Network

DING Li, CENG Shui-Ling

2018, 3(12):  18-23.  DOI: 10. 3969 / j. issn. 2096-2266.

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Aiming at the machine recognition of handwritten Xiangxi Hmong character, a classification and recognition method based
on PNN neural network was proposed. This method was improved on the basis of classical pixel projection method. In order to fully tap
the potential of the classifier and avoid over-fitting of the classifier caused by too many dimensions of characteristic matrix, text images
projected onto the pixel X axis and Y axis were classified based on great common divisors respectively to simplify the dimension of
feature matrix. In addition, feature matrix extracted by multiple pixel classification methods, combined with the training and
classification based on PNN neural network, has verified the improvement of recognition rate of the original projection method under
different pixel groups. Through experimenting on the equalization of pixels, the pixel grouping method with the best recognition rate has
been obtained.

Excellent Class Selection Method based on K-means Algorithm

CENG Xin, YANG Jian, ZHANG Xin, DAO An-Ling

2018, 3(12):  24-29.  DOI: 10. 3969 / j. issn. 2096-2266.

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The conventional selection method of excellent classes is to sum the relevant attribute values of the classes under
evaluation, and take the top K classes with a larger sum as the excellent classes. This kind of evaluation system will cause the problem
that the classes having large attribute values and having no outstanding performance in the other aspects will be rated as excellent,
whereas some classes will have an empty attribute value under the restriction of lower grades. In order to solve the existing problems,
this paper proposes an excellent class selection method based on K-means algorithm. In other words, K excellent classes with higher
similarity are selected from the samples of different grades on the basis of the clustering features that samples in the same cluster have
higher similarity and samples from different clusters have lower similarity. The results of experiments conducted using real sample data
sets indicate that: the excellent class selection method based on K-means algorithm can select outstanding classes from different
grades with balanced development of various indexes.

Design and Implementation of Medical Equipment Management Information System on Whole Life Cycle

SUN Shao-Beng, LI Yuan-Jun, ZHANG Dun, ZHANG Dun-Feng

2018, 3(12):  30-37.  DOI: 10. 3969 / j. issn. 2096-2266.

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The paper designs a simple and practical medical management system on whole life cycle in view of the present situation
and information system requirements in People's Hospital of Yuxi City. Each module of the medical equipment management system is
analyzed in detail according to the actual situation and different management demands. The system can meet the whole life cycle
management of medical equipment in People's Hospital of Yuxi City, standardize the daily work processes and improve work efficiency
through the debugging of the system.

Status Quo and Countermeasures of College Students' After-school Life

HUAN Min, MA Zhi-Min, HE Chang, LA Hai, DUAN Yi-Mei, ZUO Guo-Chao

2018, 3(12):  38-42.  DOI: 10. 3969 / j. issn. 2096-2266.

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Using stratified cluster sampling method, a questionnaire survey was conducted on 600 students from 5 majors of the
Engineering College of Dali University. Seven dimensions were investigated in the survey, including extracurricular activities,
autonomous study, social interactions and entertainment, social practice activities, physical exercise, status and opinions during the
winter and summer holidays. Through the survey, after-school life of students in Dali University is studied, their ideological trends and
spiritual life are analyzed, and countermeasures and suggestions are proposed based on the results.

Study on the Combination of Enrichment Technique and Portable Tungsten Coil Electrothermal Atomic Absorption Spectrometer

WEN Xiao-Dong, CHEN Lu-Qiong, LEI Zi-Rong, YANG Cheng-Chun

2018, 3(12):  43-48.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: In order to improve the analytical performance and expand the application scope of the new instrument
portable tungsten coil electrothermal atomic absorption spectrometer（W-coil ET-AAS）, innovative research on the combination of the
new separation and enrichment technique with the instrument was carried out, and the recent research progress was summarized.
Methods: The new separation and enrichment technique suitable for the instrument was studied and the experimental conditions were
optimized, and the analytical performance index system was established. Results: At present, a variety of simple and rapid separation
and enrichment technique has been successfully established and combined with the instrument, and its analysis performance is good.
Conclusion: Through the application of separation and enrichment technique, the analytical performance of W-coil ET-AAS has been
improved, and better detection sensitivity and enhanced anti-interference ability have been obtained.

Study on the Anti-caries Effects of Different Extracts of Geranium strictipes in vivo

LEI Yan, LI Qiu-Yan, LI Ru-Fang, LA Hai

2018, 3(12):  49-52.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To study the anti-caries effects of different extracts of Geranium strictipes R. Kunth in vivo. Methods: Models
of dental caries was induced by feeding cariogenic fodders to rats, and then ethyl acetate and n-butanol extracts from Geranium
strictipes, NaF solution, and distilled water were applied to different parts of oral cavities of the rats with a cotton swab for 5 weeks.
Finally Keyes scores were noted with a stereomicroscope, and carious surfaces were observed with a scanning electron microscope.
Results: The Keyes scores of different extracts of Geranium strictipes were lower than the distilled water group, and the difference was
statistically significant（P<0.05）; the Keyes scores of different extracts of Geranium Strictipes were higher than that of NaF group, but
the difference was not statistically significant（P>0.05）. Conclusion: Ethyl acetate and n-butanol extracts of Geranium strictipes had
anti-caries effects in vivo.

Study on the Infrared Fingerprint Spectra of Polygonatum kingianum

DAO Ai-En, DU Ze-Fei, ZHANG Cha, LI Tian, DUAN Bao-Zhong

2018, 3(12):  53-56.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To establish Fourier transform infrared spectroscopy（FTIR）spectrum of Polygonatum kingianum. Methods:
The fingerprints of P. kingianum were established by Fourier transform infrared spectrometer, and the similarity coefficient was
evaluated. Results: There were seven characteristic peaks（3 353.6 cm-1, 2 933.2 cm-1, 1 623.8 cm-1, 1 419.4 cm-1, 1 128.2 cm-1,
1 051.0 - 1, and 825.4 cm- 1） in the typical fingerprint of P. kingianum, and the similarity coefficient was greater than 0.900.
Conclusion: The method of FTIR fingerprint can be used for the quality evaluation of P. kingianum.

Simultaneous Determination of Two Anthraquinone Glucosides in Polygonum multiflorum Based on UPLC

MU Mao-Jun, LI Yang, LI Cheng-Gong, LIU Heng, TAN Feng, ZHOU Nong, ZHANG Hai-Zhu

2018, 3(12):  57-61.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To establish a simultaneous quantitative analytical method of emodin-8-O-β-D-glucoside and physcion-8-
O- β- D- glucoside in Polygonum multiflorum Thunb. based on ultra-high performance liquid chromatography（UPLC）. Methods:
The UPLC method was performance on a Waters BEH C18 column（1.7 μm, 2.1 mm × 100 mm）with a binary gradient mobile phase
consisting of a mixture of methanol and 0.1% aqueous phosphoric acid with column temperature at 30 ℃, and used a gradient elution
method. The flow rate was set at 0.2 mL/min and the detection wavelength was set at 280 nm. Results: The calibration curve for
emodin- 8- O- β- D- glucoside was linear in the range of 0.033~0.198 μg（r=0.999 5）.The average recovery of emodin- 8- O- β- Dglucoside
was 98.44%, RSD was 1.35%. The calibration curve for physcion-8-O-β-D-glucoside was linear in the range of 0.024~
0.144 μg（r=0.999 1）. The average recovery of physcion-8-O-β-D-glucoside was 95.37%, RSD was 2.41%. Emodin-8-O-β-Dglucoside
content and physcion- 8- O- β- D- glucoside content in 16 batches of Polygonum multiflorum from different regions had
significant difference. Conclusion: The UPLC method is simple, sensitive and accurate with good repeatability, which is available for
quality control of Polygonum multiflorum.

Orthogonal Test on Optimizing the Water Extraction Process of Suberville Beverage

LUO Qun, MENG Dun-Nan, BO Qing-Rong, TANG Ding-Hong, LI Nai-Yuan, YANG Sai-Jin

2018, 3(12):  62-65.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To optimize the preparation process of Suberville beverage. Methods: The extraction condition of puerarin in
the beverage was optimized by single factor method with puerarin content as the index. The yield of dry extract and the content of
puerarin were analyzed as comprehensive indicators. Orthogonal experiment was used for optimization of the water extraction process.
Results: The optimal preparation process of Suberville beverage is to add 10 times of water, decoct 3 times, and decoct for 1.5 hours
each time. Conclusion: The extraction process is objective and feasible, stable and reasonable, and it can provide theoretical basis for
process production.

Detection of Heavy Metal in Xanthium sibiricum in Yunnan

CHEN Li-Yuan, CHEN Zhi-Song, JU Min, TUN Hui

2018, 3(12):  66-69.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To detect the contents of Cd, Pb, As, Hg in Xanthium sibiricum Patr. which are grown in Dali, Baoshan,
Kunming, Wenshan, Zhaotong, Puer, Yuxi and Lincang areas respectively. Methods: The samples were nitrified and 4 kinds of heavy
metal were tested by atomic fluorescence spectrometry. Results: The heavy metal content of Xanthium sibiricum Patr. grown in
mentioned 8 areas above were in line with the standards prescribed in Green Industry Standard for Import and Export of Medicinal
Plants and Preparations. The measured elements had good linearity, r≥0.999 2, the recovery was 100.12%-100.57%, and the RSD<
5%. Conclusion: Xanthium sibiricum Patr. from 8 regions in Yunnan Province accord with Green Industry Standard for Import and
Export of Medicinal Plants and Preparations and the 2015 edition of Pharmacopoeia of the People's Republic of China. At the same
time, it provides a basis for the high-quality hierarchical management of Xanthium sibiricum Patr..

Study on Tissue Culturing of Bletilla striata

YANG Lin, LI Yu-Rong, ZHANG Wu, SHU Dan

2018, 3(12):  70-74.  DOI: 10. 3969 / j. issn. 2096-2266.

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Taking ripe capsular fruits of Bletilla striata as the test material, the rapid propagation and tissue culture were obtained by
studying the seed germination, multiplication and rooting of aseptic seedlings. Results showed that 1/2MS culture medium without
hormoneswassuitableforseedgermination.TheappropriateculturemediumforasepticseedlingmultiplicationwasMS+6-BA1.00mg/L+
NAA 0.05 mg/L+KT 1.00 mg/L and the rate of proliferation reached to 1.83. Fusarium wilt occured when tomato juice was added in the
rooting process of aseptic seedling of Bletilla striata, but the rooting rate hadn't been affected. The optimum culture medium for the
rooting of aseptic seeding of Bletilla striata was 1/2MS +NAA 1.0 mg/L+ GA3 0.5 mg/L+ tomato juice 100 g/L.

Comparative Analysis of Reproductive Performance of Landrace, Large White and Landrace×Large White Hybridization Pigs in a Large Scale Pig Farm

SU Jing-Jing, CHEN De-Fu, DUO Shi-Yan

2018, 3(12):  75-78.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To explore the reproductive performance of Landrace, Large White and Landrace×Large White Hybridization
Pigs in a large scale pig farm. Methods: SPSS 17.0 software was used to analyze the reproductive indicators. Results: There was no
statistically significant differences（P>0.05）in the litter size（LS）, numbers of alive（NAP）and healthy piglets（NHP）, and litter birth
weight（LBW）of the three types of pigs at the first parity. However, Large White and Landrace×Large White Hybridization Pigs were
significantly different from Landrace in the pregnancy periods of the first parity（P<0.01）, and there were significant differences
between Landrace and Large White in the LS（P<0.01）、NAP（P<0.01）、NHP（P<0.01）and LBW（P<0.05）at the second parity. There
were also statistical differences in the pregnancy periods between Landrace and Large White（P<0.05）, in the LS、NAP and LBW
between Large White and Landrace（P<0.01）, in the LS（P<0.05）、NAP（P<0.05）and LBW（P<0.01）between Large White and
Landrace × Large White Hybridization Pigs, and in the NHP between Large White and Landrace（P<0.05） at the third parity.
Conclusion: The reproductive indicators of different types of pigs at different parities were not consistent, with the first parity being
slightly better than the second and third parity, which may be related to the unsystematic breeding after being introduced into this farm.

Study on Artificial Propagation Technique of Schizothorax griseus

JIANG Yu-Jie, DIAO Shu-Hai, XIAO Wen, HUANG Zhi-Bang

2018, 3(12):  79-81.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To explore the feasibility of complete artificial propagation of Schizothorax griseus and to lay foundation for
large-scale artificial breeding and releasing of S. griseus, as well as the restoration of the ecological environment. Methods: Breeding
of the wild S. griseus were studied since 2011 and the artificial propagation and the fry rearing experiments were conducted in March
2012. Nearly 10 000 tail fry of the F1 generation of S. griseus were obtained. After the artificial feeding for 5 years, some F1 generation
of S. griseus successfully grew up, and dozens of the F1 generation of S. griseus were selected to procreate the F2 generation of S.
griseus by using artificial propagation techniques from March 10 to May 15, 2017. The artificial spawning method was pectoral fin
injections for two times and the average reactive time of reproduction was about 72 hours, and the water temperature for the eggs
hatching was controlled at 15 ℃. The average hatching time was 150 hours. Results: The F1 generation of S. griseus could be bred and
developed in the artificial conditions and reproduce F2 generation. F1 generation broodstock takes 3 years to mature. After the 60-day
rearing, fry of S. griseus could reach 4 cm. Conclusion: The complete artificial propagation of S. griseus can be realized.

Effects of Fire on the Structure of the Nematode-trapping Fungi Community in Cangshan Mountain

ZHOU Xin-Juan, DENG Wei, TENG Man, LIU Shuo-Ran, XIAO Wen, YANG Xiao-Yan

2018, 3(12):  82-86.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: The nematode- trapping fungi which were isolated in the burned, near- burned and non- burned soil of
Cangshan Mountain were investigated to reveal the effects of wild fire on the structure of the fungus community. Methods: A total
number of 208 soil samples were collected from the burned sites and near-burned sites of Yushan, Gantongsi and Jiangfengsi in the
north, middle and south section of Cangshan Mountain respectively, and also from non-burned sites of Lanfeng in the middle section of
Cangshan Mountain. Fungus strains were isolated and purified by traditional methods, identified by morphological and molecular
methods, and classified according to the new classification system. Results: A total number of 150 nematode-trapping fungus strains
were isolated from the 208 soil samples and classified into 18 species in 3 genera, including 1 species to be identified. The detection
rates of genus Dactylellina-Drechslerella-Athrobotrys in burned sites, near-burned sites and non-burned sites were 67.39%, 20.65%,
11.96%, 52.94%, 8.82%, 38.24%, 29.16%, 0.00%, 70.84% respectively. The dominant species in the burned sites, near-burned sites
and non-burned sites were Dactylellina ellipspora（OF=36.59%）, Dactylellina ellipspspora（OF=16.36%）, Dactylellina drechsleri
（OF=16.36%）and Arthrobotrys oligospora（OF=30.00%）respectively. The diversity indexes H' were 2.05, 2.35 and 1.82, respectively.
Conclusion: Burning in the forest will significantly alter the community structure of nematode-trapping fungi in soil, meanwhile,
which could promote the succession of nematode-trapping fungi community.

Distribution Pattern of Nematode-trapping Fungi in Eastern and Western Slopes of
Northern Gaoligong Mountain

WANG Jia-Liang, LIU Shuo-Ran, ZHOU Han-Yu, FANG Si-Hao, XIAO Wen, YANG Xiao-Yan

2018, 3(12):  87-91.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To investigate the distribution pattern of the nematode-trapping fungi in eastern and western slopes of northern
Gaoligong Mountain, and to study the effects of slope direction on the spatial distribution of nematode-trapping fungi. Methods: Soil
samples were collected from the east and west slopes. The fungi strains were identified morphologically and molecularly, and then, the
detection rate and species richness were set as biological indices which was analyzed. Results: Firstly, the abundance of nematodetrapping
fungi in the west slope was higher than the east slope. Secondly, the species richness and the detection rate of nematodetrapping
fungi in the two slopes showed a trend of ascending firstly and then descending with the elevating of slope position. The
dominant species in both slopes were Arthrobotrys thaumasium. Conclusion: The distribution pattern of the nematode-trapping fungi
was heterogeneous between eastern and western slopes of northern Gaoligong Mountain, and the different temperature and rainfall
between the slopes might be the main factors causing the heterogeneity.

Resourses Investigation on Lignicolous Freshwater Hyphomycetes of Jingsha River

LI Wen-Li, CHEN Hong-Wei, ZHANG Yan-Mei, RUAN Qing-Xiong, ZHOU Ji-Shan, SU Hong-Yan

2018, 3(12):  92-97.  DOI: 10. 3969 / j. issn. 2096-2266.

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Objective: To investigate the diversity characteristics and community composition of lignicolous freshwater hyphomycetes
in high altitude section of Jinsha river. Methods: 200 samples of decaying wood were collected from Jinsha river (Shigu Town - Baima
Snow Mountain section, altitude: 1 814-3 034m). After 1-2 weeks of moisturizing culture, lignicolous freshwater hyphomycetes were
isolated and purified. Based on morphological and molecular biological methods, lignicolous freshwater hyphomycetes were classified
and identified. Results: A total of 55 lignicolous freshwater hyphomycetes were isolated and 37 species of 23 genera were identified, of
which Minimelanolocus, Stachybotrys and Bactrodesmium had relatively high occurrence frequency. The diversity index was 3.48. The
diversity indexes of lignicolous freshwater hyphomycetes from 1 800-1 900 m, 1 900-2 000 m, 2 000-3 000 m and above 3 000 m
were 2.07, 2.01, 1.57 and 1.50, respectively. Conclusion: ①In the high altitude section of Jinsha river (1 814-3 034 m), the diversity
of lignicolous freshwater hyphomycetes is rich. ② With the increase of altitude, the diversity of lignicolous freshwater hyphomycetes
decreases gradually in the high altitude section.

Analysis of Features of Highly-cited Papers in Journal of Dali University  

LI Yang

2018, 3(12):  98-102.  DOI: 10. 3969 / j. issn. 2096-2266.

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Through analyzing the features of highly-cited papers published in Journal of Dali University, this paper intends to
understand its development status, and then proposes improvement measures to promote the healthy and sustainable development of
the journal. The results showed that the highly-cited papers had a low positive correlation with the frequency of downloading（r=0.637,
P=0.000）. In the cross-database search results of China National Knowledge Internet, the total number of citations from academic
journal databases and master thesis databases was the highest（both were 850 times）. The maximum number of citations for a single
paper in academic journal databases was 42, and that in master thesis databases was 97. Statistics on citation regions and citation units
revealed that a total number of 250 units distributed in 31 provinces（cities）across the country had cited from Journal of Dali
University. Through the statistical classification of citation units, it can be seen that they are mostly from the normal and the medical
organizations.