Table of Content

15 April 2020, Volume 5 Issue 4

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Analysis of Hepatotoxicity Mechanism of Bavachinin Based on Label-Free Technique

Zhu Yue, Wang Shan, Xu Lijiao, Sun Xiaobo, Ji Yubin, Sun Guibo

2020, 5(4):  1.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 001

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〔Abstract〕Objective: To study the toxicity of bavachinin on HepaRG cells and to lay a foundation for elucidating the hepatotoxicity
mechanism of bavachinin. Methods: Label-free quantitative proteome technique was used to extract and analyze the protein difference
between the drug administration group and the control group, and the KEGG signal pathway analysis was taken on differential proteins.
Results: Bavachinin could induce the expression and differentiation of many proteins in HepaRG cells, including 372 differentially
expressed proteins, 170 upregulated proteins and 202 downregulated proteins. KEGG signal pathway analysis showed that there were a
large number of protein changes in the metabolic pathway. Conclusion: Bavachinin may induce hepatotoxicity by affecting the
expression of genes in cellular metabolic pathway.

Effects of Scutellarein on the Tension of Isolated Guinea Pig Bronchial Smooth Muscle and Its Mechanism

Hu Can, Zhang Yiming, Miao Zhimin, Lai Yong

2020, 5(4):  7.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 002

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〔Abstract〕Objective: To investigate the effects of scutellarein on normal and multiple spasmogen-induced isolated guinea pig
bronchial smooth muscle and its mechanism of action. Methods: Bronchial smooth muscle spiral strips of guinea pigs were prepared,
and the effects of scutellarein on its tension were observed under physiological conditions, with and without calcium, respectively after
treatment with histamine diphosphate, pilocarpine and potassium chloride. Results: 0.038-0.610 g/L scutellarein had a significant
relaxant effect on bronchial smooth muscle contraction induced by histamine diphosphate and pilocarpine（P<0.05）, and had no
obvious relaxant effect on bronchial smooth muscle contraction induced by potassium chloride（P＞0.05）. Conclusion: Scutellarein
has a certain relaxant effect on isolated bronchial smooth muscle of guinea pigs, and its mechanism may be associated with the blocking
of histamine receptor and nicotinic acetylcholine receptor.

Study on Oxidative Damage and Hcy Level in Rat HIRI Model

LuJiansheng,Tian Xinyan,SuJuan,L ü Xing,WangYongkuan, PangBo,ZhouPing

2020, 5(4):  12.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 003

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〔Abstract〕Objective: To study the changes of oxidative injury and homocysteine（Hcy）content in the hepatic ischemia-reperfusion
injury（HIRI）model of rats. Methods: Hepatic arteries and portal veins of the left lateral lobe and middle lobe of the rat liver were
clamped with non-invasive vascular clamps, and about 70% of the blood flow to the liver was blocked to establish a rat HIRI model. The
rats in Sham groups（S-I group and S-I/R group）were only intervened at the hepatic pedicle. The rats were sacrificed after 0 h（S-I
group and I group） or 24 h （S-I/R group and I/R group）. The activities of aspartate aminotransferase （AST） and alanine
aminotransferase（ALT）in plasma were measured. Superoxide dismutase（SOD）activity and the contents of malondialdehyde（MDA）,
homocysteine（Hcy）, hyaluronic acid（HA）and laminin（LN）were measured. The pathological changes of liver tissue were observed by
hematoxylin-eosin staining. Results: Compared with the S-I/R group, the hepatocytes of the I/R group showed obvious degeneration
and necrosis, the plasma AST and ALT activities increased（P<0.01）, and the contents of MDA, HA, LN and Hcy were increased in
liver tissue（P<0.05）. The activity of SOD decreased（P<0.01）. Conclusion: Hepatic ischemia-reperfusion can induce liver injury
through oxidative stress, the content of Hcy was increased, and Hcy may be involved in the occurrence of hepatic ischemia-reperfusion.


Effects of Ginsenoside Rg1 on the Proliferation of K562 Cells

Han Yanjun, Wang Cuili, Liu Xiaohu, Zhou Yue

2020, 5(4):  16.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 004

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〔Abstract〕Objective: To investigate the effects of Ginsenoside Rg1 on the proliferation of K562 cells. Methods: K562 cells were
treated with different concentrations of Ginsenoside Rg1. The effects of Ginsenoside Rg1 on proliferation of K562 cells were detected
by CCK-8 assay and colony formation assay. The cell cycle of K562 was detected by flow cytometry. Results: Ginsenoside Rg1 could
effectively inhibit the proliferation of K562 cells, decrease the colony formation rate of K562 cells, inhibit K562 cells from entering S
phase, and block G 0 /G 1 phase. The most potent inhibitory effect on K562 cell proliferation was 20 μmol/L, and the optimal duration of
action was 48 h. Conclusion: Ginsenoside Rg1 can effectively inhibit the proliferation of K562 cells.


Optimized Extraction Process of Qianhua Preparation by OrthogonalDesign with Pharmacodynamics Index

Huang Rui, Yuan Yuan, Ren Guilin, Zhao Jian, Yang Sijin, Li Zhi, Pu Qingrong

2020, 5(4):  21.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 005

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〔Abstract〕Objective: To optimize the extraction process for Qianhua Preparation. Methods: Rat model of hyperuricemia induced by
yeast extract and adenine was used to compare the indexes of different processes of Qianhua Preparation. Taking rubimaillin as index,
the effects of particle size, ethanol dosage, soaking time and precolate speed on the extraction process of Qianhua Preparation were
investigated by orthogonal test. Results: Optimum extraction process was to crush the drug through No. five sieve and add 8 times the
amount of 65% ethanol to soak for 24 hours with percolation speed of 2 mL/min. Conclusion: The optimized extraction process of
Qianhua Preparation is reasonable, stable with higher extraction rate.


Effects of Methadone on the Behaviors and Intestinal Microflora in Mice

Deng Qilei, Zhang Min, Li Hongliu, Han Tingting, Shen Yuanying

2020, 5(4):  25.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 006

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〔Abstract〕Objective: To analyze the effects of methadone on the behaviors and intestinal microflora in mice with normal flora and
dysfunctional flora. Methods: Four groups of mice were treated with gavage, and the tail suspension test and the open field test were
tested after 28 days. The SIgA content in the intestinal tissue was determined by ELISA, and the colony was collected from the mouse
feces using the selection medium. Results: The SIgA content in the intestinal tissues of the 4 groups of mice, the static immobility time
after the suspension tail test, the number of uprights in the open field test, the number of aerobic bacteria and yeast colonies were
statistically significant（P<0.05）, and the SIgA content in the intestinal tissue has a positive correlation between the number of yeast
colonies（r=0.248）. Conclusion: Methadone has an effect on the composition of the intestinal flora and the local immune function of
the intestinal mucosa. The aerobic bacteria and yeast are significantly reduced, and the local immune function of the intestinal mucosa
is decreased and may be related to the number of intestinal yeast. Methadone has an effect on the behavior of mice in dysbacteriosis,
with less depression and greater adaptation to the environment.


Correlation between the Polymorphism of SNP rs4938723 in miR-34b/c Gene and Oligospermia

Ke Hongli, A Zhoucun

2020, 5(4):  30.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 007

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〔Abstract〕Objective: To investigate the correlation between the polymorphism of SNP rs4938723 in miR-34b/c gene and oligospermia.
Methods: The polymorphism distribution of rs4938723 was investigated by using polymerase chain reaction and restriction fragment
length polymorphism assay in 273 patients with oligospermia and 234 controls. Results: The frequencies of allele C（35.0% vs. 28.2%,
P=0.021, OR=1.378, 95% CI is 1.048-1.789）and genotype CC（13.9% vs. 7.3%, P=0.016, OR=2.064, 95% CI is 1.132-3.764）were
significantly higher in patients with oligospermia than those in the control group. Conclusion: The polymorphism of SNP rs4938723 in
miR-34b/c gene may be associated with oligospermia, and genotype CC of SNP rs4938723 may increase the risk of oligospermia.


In vitro Culture Mouse Embryo Assay for Evaluating the Quality Control of IVF Laboratory

Peng Tao, Liu Yonggang, Li Qin

2020, 5(4):  34.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 008

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〔Abstract〕Objective: Before the new IVF laboratory is put into operation, quality control of laboratory environment, incubator and
personnel operation should be carried out in time. The results of quality control should be evaluated by in vitro culture of mouse
embryos, which can be used only after qualified. Methods: Detecting the VOC after heat ventilation treatment; using the blood gas
analyzer to detect the culture solution to set the incubator; verifying the survival test of human sperm in vitro; obtaining the male and
female gametes of Kunming mice, and in vitro fertilization culture to observe the fertilization rate and cleavage of the mouse embryo
rate, blastocyst rate; if the ratio of 2-cell embryo development to blastocyst is ≥80%, the test is qualified. Results: The environmental
values of testing laboratories are in line with the standards; the incubator with 7% CO 2 concentration can be used normally; the in vitro
fertilization rate was 80.9%, the cleavage rate was 97.6%, and the blastocyst rate was 87.1%. Conclusion: Laboratory quality control
method is effective, and IVF laboratory can be put into use.


Thought of the Practice of Bilingual Teaching Extension Model in Medical Microbiology

Wu Lixian, Shen Yuanying, Zhang Lei, Wu Youcong, Liu Qi

2020, 5(4):  39.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 009

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〔Abstract〕Objective: To explore the bilingual teaching model of Medical Microbiology, and to apply it in teaching practice. Methods:
The clinical medicine specialty was taken as a pilot, and the proportion of bilingual teaching had been increased year by year for 8 years,
and the teaching model had been extended to the English teaching of South Asian students. Results: Through the practice of bilingual
teaching model of Medical Microbiology, the students changed from "passively listening" to "actively learning", which not only improved
the bilingual teaching effects of medical courses, stimulated the students' learning enthusiasm, but also cultivated excellent teachers.
Conclusion: The bilingual teaching model of Medical Microbiology has achieved good results.


Practice and Research on the Construction of Teaching Resource Base of#br# Morphological Virtual Network Platform

Shi Yiling, Chen Haibin, Li Yan, Yang Wenjuan

2020, 5(4):  43.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 010

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〔Abstract〕Objective: With the continuous development of information technology, virtual network teaching is used in experiment
teaching in medical schools. In order to improve the information-based experiment teaching and realize the teaching model of "Internet
+", the first step is to build a teaching resource database. Resource platform construction is the foundation of the virtual network
teaching. Methods: Through the collection of the digital data of the pathological experimental teaching gross specimens, together with
the now used virtual section network teaching resources and the internet multimedia interactive system, after the three-dimensional
processing of specimen images were uploaded to the network, the construction of a complete morphological virtual network platform was
achieved. Results: The level and quality of Pathology experimental teaching were improved, and internet-based experimental teaching
and virtual teaching were realized under the background of big data era. Conclusion：Experimental virtual network teaching is the
trend of the times and an inevitable development of the information age background. We further network teaching resources practice
and research based on the experimental teaching.


Effects of Pulmonary Lobectomy on the Right Ventricular Function and the Living Quality#br# of Patients with Non-Small Cell Lung Cancer

YangWenrong,LiZhuang,LiAimin,YangLijie,ChaJianyuan

2020, 5(4):  47.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 011

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〔Abstract〕Objective: To discuss the effects of pulmonary lobectomy on the right ventricular function and the living quality of
patients with non-small cell lung cancer. Methods: 102 patients with non-small cell lung cancer were selected from June 2013 to June
2018 in the First Affiliated Hospital of Dali University. According to the operation method, all patients were divided into lung lobe
group（50 cases）and whole lung group（52 cases）, the former having received the treatment with lobectomy of lung, and the latter
having received the treatment with pneumonectomy. Results: The time of operations and the lymph node numbers of the lung lobe
group were almost the same with the whole lung group, and the difference was not statistically significant（P＞0.05）; the intraoperative
blood loss and the time of postoperative hospitalization of the lung lobe group were significantly lower than those of the whole lung
group, and the difference was statistically significant（P < 0.05）; after the operations, the forced vital capacity（FVC）, the forced
expiratory volume（FEV）and the carbon dioxide dispersion（DLCO）of the lung lobe group were significantly higher than those of the
whole lung group, and the difference was statistically significant（P < 0.05）; after the operations, the heart rate（HR）and the right
ventricular diastolic volume index（RVEDVI）of the lung lobe group were significantly lower than those of the whole lung group; after
the operation, the right ventricular ejection fraction（RVEF）of the lung lobe group was significantly higher than that of whole lung
group, and the difference was statistically significant（P < 0.05）; in one month and three months after the operations, the Lung Cancer
Symptom Scale（LCSS）score of the lung lobe group was significantly lower than that of the whole lung group, and the difference was
statistically significant（P < 0.05）. Conclusion: Pulmonary lobectomy is an effective treatment for the non-small cell lung cancer. It
can promote the recovery of pulmonary function and decrease the adverse effect on right cardiac function, which can improve the
quality of life.


Retroperitoneal Cystic Lymphangioma: A Case Report and Literature Review

Gao Long, Lei Yi

2020, 5(4):  52.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 012

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〔Abstract〕Objective: To discuss the disease characteristics of retroperitoneal cystic lymphangioma and to improve the diagnosis and
treatment of retroperitoneal cystic lymphangioma. Methods: Clinical data of 1 case of retroperitoneal cystic were reported, and image
findings were retrospective analyzed. Results: A 4-year-old male was admitted to hospital for 3 days due to abdominal pain. Physical
examination: there was no bulge in the bilateral kidney area, no redness and swelling in the skin, both in the supine position and in the
sitting position, both the kidneys were touched, the abdomen was flat, with left abdominal tenderness and slight muscular guard. A
lump of about 12 cm × 12 cm was palpable, with unclear quality and boundary, not easy to push and tenderness was obvious. Resection
of left retroperitoneal mass was performed. Postoperative pathological diagnosis: retroperitoneal cystic lymphangioma. Follow-up for 3
years showed no recurrence. Conclusion: The epidemiology, clinical manifestation, diagnosis and treatment of the disease were
analyzed in combination with previous literature. Retroperitoneal cystic lymphangioma is a rare benign lesion, but its growth pattern is
aggressive, and there are many kinds of treatment methods. Complete resection of the lession is an effective method for the treatment of
the disease. There recurrence rate is low and the prognosis is better.


The Relationship between miR-149 and miR-196a2 Gene Polymorphism and Stroke in Asian:#br# A Meta-Analysis

Yang Xitong, Xu Hongyang, Ma Rong, Wang Guangming

2020, 5(4):  56.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 013

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〔Abstract〕Objective: To investigate the association between miR-149, miR-196a2 gene polymorphism and ischemic stroke（IS）in
Asian. Methods: From the PubMed, Web of Science、CBM、CNKI, Wanfang and Weipu Datebase, case-control studies about the
association between microRNAs gene polymorphism and ischemic stroke were collected from the inception to November 2018. Two
reviewers independently screened literature, extracted data and assessed the risk of bias of included studies. Then meta analysis was
performed with Stata 12.0 software. Results: A total 6 472 cases of eight case-control studies, including 3 332 patients of IS and 3 140
of the control group were included in this study. The meta-analysis showed the association between miR-149 gene rs2292832
polymorphism and the risk of ischemic stroke. Results indicate that C allele and CC genotype of miR-149 rs2292832 are associated
with significantly higher risks of IS ,［C vs. T: OR=1.40, 95%CI（1.01, 1.93）, P=0.04; CC vs. TT: OR=1.31, 95%CI（1.09, 1.57）, P=
0.004; CC vs. CT+TT: OR=1.29, 95%CI（1.09, 1.53）, P=0.003］. Conclusion: miR-149 gene polymorphism is correlated with IS in
Asian, C allele and CC genotype of miR-149 rs2292832 are associated with significantly higher risks of IS, and no significant
associations were found between miR-196a2 rs11614913 and IS susceptibility in the studies.


Diagnostic Value of Ultrasound-Guided Fine Needle Aspiration Biopsy for TI-RADS 4-5 Nodules

Li Xin, Wang Jing, Chen Haiyan, Liu Fang, Liu Qingqing

2020, 5(4):  65.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 014

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〔Abstract〕Objective: To investigate the diagnostic value of ultrasound-guided fine needle aspiration biopsy（US-FNAB）for benign
and malignant thyroid TI-RADS 4-5 types of nodules. Methods: A retrospective analysis was performed on 173 patients with TI-
RADS category 4-5 thyroid nodule who underwent US-FNAB examination in the First Affiliated Hospital of Dali University from
January 2017 to March 2019 and postoperative pathological results were obtained, including 235 nodules. Nodules were divided into
the following four groups: TI-RADS 4A, TI-RADS 4B, TI-RADS 4C and TI-RADS 5. Postoperative pathology was used as the gold
standard, and the correlation between US-FNAB cytology and postoperative pathology was evaluated by χ
2
text. The specificity,
sensitivity, negative predictive value and positive predictive value of US-FNAB were calculated. Results: The accuracy of cytological
results of nodules US-FNAB in the four groups was compared with the postoperative pathological results, and had no statistical
significance（P>0.05）. The sensitivity, specificity, positive predictive value and negative predictive value of TI-RADS 4-5 nodules of
US-FNAB were 95.74%, 91.49%, 97.83% and 84.31%, respectively. Conclusion: US-FNAB has high diagnostic value for TI-RADS
4-5 thyroid nodules, which can be used as an effective method for the preoperative diagnosis of thyroid nodules.


Clinical Value of TVS 4D-Hycosy in the Diagnosis of Infertility

Xia Qian, Li Yulan, Ma Hongmei, Chen Haiyan, Liu Fang, Liu Qingqing

2020, 5(4):  69.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 015

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〔Abstract〕Objective: To explore the clinical value of transvaginal four-dimensional hysterosalpingo-contrast sonography（TVS 4D-
Hycosy）in the diagnosis of infertility. Methods: 324 cases were diagnosed with infertility in the First Affiliated Hospital of Dali
University from January 2018 to December 2018, and TVS 4D-hycosy was adopted to observe the patency of fallopian tube and uterine
cavity lesions. Results: Among the 324 patients, there were 125 cases of bilateral oviduct patency, 46 cases of bilateral obstruction,
127 cases of unilateral obstruction, 26 cases of unilateral or bilateral partially obstruction, 11 cases of endometrial polyps, 5 cases of
intrauterine adhesion, 1 case of submucosal fibroids; 6 cases of fallopian tube hydrops, 6 cases of cervical adhesion, 2 cases of uterine
deformity, and 37 cases with contrast agent myometrial countercurrent. 93 cases were pregnant within 6 months of follow-up.
Conclusion: TVS 4D-Hycosy is capable of real-time dynamic judgment tubal patency and uterine cavity lesions, and it has the
advantages of high accuracy, simple operation, noninvasive, no radiation, less adverse reaction, and strong repeatability, etc. At the
same time, it has the dual role of diagnosis and treatment, therefore it is a kind of examination of uterine cavity lesions of tubal patency
and effective method which is worthy of being widely used clinically.


Correlation between Serum Uric Acid and Metabolism of Glucose and Lipid in Middle-Aged#br# and Elderly Patients with Type 2 Diabetes

Zhu Li, Qiu Hongmei

2020, 5(4):  73.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 016

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〔Abstract〕Objective: To investigate the correlation between serum uric acid（SUA）and glycolipids metabolism in middle-aged and
elderly patients with type 2 diabetes mellitus（T2DM）. Methods: In this study, 845 patients with T2DM aged over 40 in the
People's Hospital of Yuxi City were selected and divided into 5 groups according to the level of the glycosylated hemoglobin（HbA 1 c）,
group A with HbA 1 c < 7.0%, group B with 7.0%≤HbA 1 c＜9.0%, group C with 9.0%≤HbA 1 c＜11.0%, group D with 11.0%≤HbA 1 c＜
13.0%, and group E with HbA 1 c≥13.0%, to compare the prevalence of hyperuricemia in 5 groups and analyze the correlation between
SUA level and various metabolic indexes. Results: The prevalence of hyperuricemia in 5 groups gradually decreased with the increase
of HbA 1 c level, with significant statistical differences（P<0.05）. Multiple regression analysis showed that the level of blood uric acid
was positively correlated with gender（male/female）, weight, total cholesterol（TC）, triglycerides（TG）, and low-density lipoprotein
cholesterol（LDL-C）（P<0.05）, and was negatively correlated with age, HbA 1 c, and high-density lipoprotein（HDL-C）（P<0.05）.
Conclusion: SUA levels in type 2 diabetes patients are closely related to metabolic indicators such as blood sugar control level, insulin
resistance, and lipid metabolism disorder.


Analysis on the Consultation Cases Participated by Clinical Pharmacists in a Hospistal#br# in the Last Three Years

Wang Li, Chen Yong, Gao Yan, Zhou Yuan, Yang Chunsong

2020, 5(4):  78.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 017

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〔Abstract〕Objective: To analyze the situation and characteristics of clinical pharmacists involved in consultation cases, and to
improve the consultation level of clinical pharmacists so as to provide reference for further development of clinical pharmaceutical
work. Methods: A retrospective analysis of 877 clinical consultation cases involved clinical pharmacists in the People's Hospital of
Yuxi City from 2016 to 2018 was made, including consultation form, department applying for consultation, consultation purpose,
opinion adoption, etc. Results: Of the 877 consultation cases, general consultation was the main form（87.80%）, the second form was
the hospital consultation（9.58%）, followed by emergency consultation（2.62%）; 28 departments were involved, including the medical
departments and the surgery departments, among them, there were more consultation cases in surgical departments（81.41%）; anti-
infection treatment was the main consultation purpose（91.22%）; the adoption rate of the clinical pharmacist suggestions was 92.93%,
including full and partial adoption. Conclusion: The needs for clinical pharmacists to participate in clinical consultation are increasing,
and have achieved certain results, but we still need to improve the consultation ability in order to better promote rational drug
application.


Application of Multiple Teaching Methods in Clinical Novitiate of Neurology

Dong Zimei, Li Zhihong

2020, 5(4):  82.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 018

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〔Abstract〕Objective: To improve the clinical practice ability and comprehensive quality of students. Methods: The problem-based
learning teaching method（PBL）, case-based learning teaching method（CBL）, and team-based learning teaching method（TBL）were
adopted to improve students' learning interest and cultivate their comprehensive ability. Results: As the supplementary of lecture-
based learning teaching method（LBL）, each of the adopted methods has its own advantages and disadvantages in improving learning
interest and cultivating comprehensive ability. Conclusion: The application of the three teaching methods allows students to be
problem-oriented and case-based, to participate in the clinic "immersively" in the team, fully mobilize the students' learning interest,
and improve their clinical practice ability and comprehensive qualities.


A Meta-Analysis of the Effects of Environmental Lead Exposure on Children's Intelligence

Qu Tengjiao, Sun Mingjun, Gao Zhangjin, Zhao Qingyun, He Zuoshun

2020, 5(4):  85.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 019

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〔Abstract〕Objective: Using the Meta-analysis methods commonly applied in medicine to comprehensively analyze and explore the
effectsofleadexposureonchildren'smentaldevelopment.Methods:BasedonChineseorEnglishkeywords"lead""child" "intellectual",
articles about the influence of lead exposure on children's nerves or intelligences were searched though various databases（CNKI,
Weipu, Wanfang, PubMed and Medline）. After a quality evaluation of the literature, the heterogeneity analysis, sensitivity analysis and
stratification analysis of the included literature were carried out using Review Manager 5.3. Results: A total of 14 articles（1 article in
English and 13 articles in Chinese）were collected according to the pre-designed screening criteria. The analysis showed that there
was a high degree of heterogeneity among the studies（I
2 >50%）, and the random effects model was selected to analyze the mean
difference (MD) and 95% confidence interval of full intelligence quotient（FIQ）, verbal intelligence quotient （VIQ）, and performance
intelligence quotient（PIQ）. Results showed that the study outcome was P<0.001 and within the 95% confidence interval. The PIQ
indicators were eliminated from time order of publication and from large to small in accordance with the sample size, and the VIQ
indicators were eliminated from large to small in accordance with the sample size. The results showed the reliability of the combined
effect, indicating that the combined results were statistically significant. Finally, a subgroup analysis of the research site was carried
out, and it was found that the field study was the influencing factor of the heterogeneity of each indicator.Conclusion: Lead exposure
has a negative impact on children's neurological and intellectual development.


Investigation on Pain Perception of Clinicians in County General Hospitals

Zhong Qiong, Dong Shaoxing, Liu Jianping, Cai Defang

2020, 5(4):  92.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 020

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〔Abstract〕Objective: To investigate the current status of pain perception（knowledge and attitude）among clinical pain rehabilitation
doctors in county general hospitals in Yuxi City, and to analyze the influencing factors. Methods: By issuing questionnaires and
Wisconsin Pain Knowledge Scale, 65 clinical pain rehabilitation doctors in 9 county general hospitals of 2 districts and 7 counties in
Yuxi City were surveyed about the status of pain recognition, and the influencing factors were analyzed. Results: The knowledge about
pains of clinical pain rehabilitation doctors in Yuxi county general hospitals was insufficient, with only an average accuracy rate of
48.33% in the questionnaire. The accuracy rate of knowledge of pain assessment was only 21.54%. Factors such as educational
background, gender and professional title had no effect on the overall score. Doctors' attitude towards analgesia was vague. Only 27.69%
of the doctors followed the three-step analgesia plan completely. Conclusion: Clinical pain rehabilitation doctors lack knowledge of
pain, especially in pain assessment and intervention. They are not clear about whether to adopt the three-step analgesia. Therefore, only
by strengthening the standardized management of pain assessment can the patients' medical experience be improved.


Research on the Effects of Occupational Protection Compensation Education for Higher Vocational#br# Nursing Students before Practice

Zhuang Lijuan, Zhu Weibo, Zhang Shufang, Yan Xue, Ma Yunchun, Zhu Hongfen

2020, 5(4):  96.  DOI: ［DOI］10. 3969 / j. issn. 2096-2266. 2020. 04. 021

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〔Abstract〕Objective: To explore the contents and effects of occupational protection compensation education for higher vocational
nursing students before practice. Methods: Using self-designed questionnaire to investigate the nursing occupational protection
awareness and knowledge of 221 higher vocational nursing students. According to the results, the contents and methods of occupational
protection compensation education were determined. And then another 223 higher vocational nursing students were given occupational
protection compensation education before practice. At last, mastery of the knowledge of occupational protection, occupational protection
awareness and occupational exposure incidence between the two groups were compared. Results: ① After receiving compensation
education, the mastery of occupational protection knowledge of the experimental group was better than that before education（P<0.01）;
② the knowledge and awareness of occupational protection of nursing students in the experimental group were better than those in the
control group（P<0.01）; ③ the incidences of physical, chemical and biological occupational exposure of the nursing students in the
experimental group were lower than those of the control group（P<0.05）. Conclusion: The development of occupational protection
compensation education before practice is beneficial for strengthening the knowledge of occupational protection of nursing students, for
it can improve the awareness of occupational protection and reduce occupational injury, thus promoting the physical and mental health
and smooth employment of nursing students.