Journal of Dali University

The Protective Effect and Mechanism of Panax notoginseng Saponins on ox-LDL-Induced Platelet Apoptosis

Ma Yongjie, Li Weiqi, Wu Chunting, Huang Xinhui, Zhou Xinyu, Ya Fuli,

2023, 8(2): 1-7.

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Objective： To explore the effect of Panax notoginseng saponins（PNS） on oxidized low-density lipoprotein（ox-LDL）-induced platelet apoptosis and its potential molecular mechanism in vitro. Methods: Human gel-filtered platelets were pre-treated with different concentrations of PNS （0，1，10，100 μg/mL） for 40 minutes， and then stimulated with 50 μg/mL ox-LDL for 60 minutes. Subsequently， indicators of platelet apoptosis including platelet mitochondrial membrane potential（Δψm） levels and phosphatidylserine（PS） exposure levels were detected by flow cytometry. Reactive oxygen species（ROS） levels and superoxide levels were measured by commercial ROS assay kit. The phosphorylation levels of Src and Syk proteins as well as the activation levels of Caspase-3 and Caspase-9 were detected by Western blotting. Results: Compared with the control group， ox-LDL-induced depolarization of platelets Δψm was significantly reduced in each dose group of PNS（P<0.01）， ox-LDL-induced exposure level of platelet surface PS（P<0.05）， total ROS levels and superoxide levels（P<0.05）， Caspase-3 and Caspase-9 activation levels（P<0.05），and phosphorylation levels of Src and Syk proteins（P<0.05） were inhibited in the medium and highdose PNS groups. Conclusion：PNS may play the role of anti ox-LDL-induced platelet apoptosis by inhibiting Src/Syk/ROS signalling pathway.

Study on the Mechanism of Compound Longxuejie Capsules in Treating Coronary Heart Disease Based on Network Pharmacology and Molecular Docking

Zhu Qianwei, Yang Hua, Qian Kai, Li Dan, Xia Conglong

2023, 8(2): 8-18.

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Objective： To explore the mechanism of Compound Longxuejie Capsules in treating coronary heart disease by network pharmacology and molecular docking. Methods： The chemical components of Compound Longxuejie Capsules were searched by Traditional Chinese Medicine Systems Pharmacology database； SwissADME platform and Swiss Target Prediction database were used to screen active components and obtain drug targets； disease targets were obtained from GeneCards and other databases； drug-component-disease target network and the protein-protein interaction network were constructed； GO analysis and KEGG pathway enrichment analysis were conducted through Metascape platform； AutodockVina software was used to dock the active components with the key targets. Results： 81 active components were screened. 124 targets were related to coronary heart disease， and the key targets were AKT1， EGFR， AR， PTGS2， SRC and ESR1. GO analysis and KEGG pathway enrichment analysis showed that the targets involved nuclear factor-κB， PPAR and other signaling pathways， and biological processes such as inflammation reaction and fatty acid metabolism. The results of molecular docking showed that the binding activity of the active component to the key targets was strong. Conclusion： It is predicted that quercetin， resveratrol and other active components in Compound Longxuejie Capsules will combine with AKT1， AR， PTGS2 and other targets to act on nuclear factor-κB， PPAR and other signal pathways， which play a role in treating coronary heart disease.

Inhibition of K562 Cell Proliferation by p53 Protein Regulated by Periplaneta americana Extract CⅡ-3

He Siyue, Tang Ziyun, Liu Heng, Zhang Chenggui, Zhou Wen, Bi Ziying, Zhou Yue

2023, 8(2): 19-23.

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Objective： To investigate the effect of Periplaneta americana extract CⅡ-3 on the proliferation of human leukemia cell K562. Methods： K562 cells were treated with different concentrations of Periplaneta americana extract CⅡ-3， and the effects of CⅡ-3 on the proliferation of K562 cells were detected by CCK-8 assay. Colony formation assay was used to detect its effect on proliferation of K562 cells. The cell cycle of K562 cells was detected by flow cytometry. The expression of phosphorylated p53 protein was detected by Western blot. Results：Periplaneta americana extract CⅡ-3 could inhibit the proliferation of K562 cells， and the optimal concentration and duration of action were 80 μg/mL and 72 h respectively. Periplaneta americana extract CⅡ-3 reduced the colony formation rate of K562 cells. The proportion of K562 cells blocked in G0/G1 phase increased， while that in S phase decreased. The expression of phosphorylated p53 protein was up-regulated. Conclusion： Periplaneta americana extract CⅡ-3 inhibits the proliferation of K562 cells by regulating p53 protein.

Bioinformatics-Ｂased Analysis and Screening of Differentially Expressed Genes in Pancreatic Cancer

Li Weiqian, Chen Yiming, Zhang Wenting, Su Yingzhen, Shuai Hongyan, Yu Xin

2023, 8(2): 24-30.

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Objective：To analyze the information of GEO database by bioinformatics， and explore the key genes and signal pathways of pancreatic cancer. Methods：The gene expression profile chip GSE107610 of pancreatic cancer was downloaded， and the differentially expressed genes were statistically analyzed by R software， so as to study the related biological processes， signal pathways and protein-protein interaction. The protein-protein interaction network diagram was constructed by Cytoscape software， the differences of samples were verified by hematoxylin and eosin staining， and the four core genes screened out were verified by real-time fluorescence quantitative polymerase chain reaction. Results：Among 71 differentially expressed genes， 17 were up-regulated and 54 were down-regulated. The functions of these genes were predominantly involved in protein hydrolysis， alcohol metabolism， exogenous metabolism， digestion， immune response regulation and other processes. The results of the GEO database analysis were consistent with the expression of key genes at the mRNA levels. Conclusion：Using bioinformatics technology， the differentially expressed genes related to the occurrence and development of pancreatic cancer have been successfully discovered. It has been verified that the expression levels of the above differentially expressed genes are consistent in human pancreatic cancer tissues. This study provides a new idea for determining early diagnostic markers and therapeutic targets of pancreatic cancer.

Bioinformatic Analysis of Differentially Expressed Genes in the Patients Infected with Helicobacter pylori

Cheng Mingjing, Yan Ping, Fan Yujuan, Zhao Weidong

2023, 8(2): 31-35.

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Objective：To investigate the differentially expressed genes （DEGs） in the patients infected with Helicobacter pylori （H. pylori）. Methods： Three H. pylori infection datasets （GSE6143， GSE60427 and GSE60662） were collected from Gene Expression Omnibus database. The common DEGs in the three data sets were analyzed by bioinformatics software for KEGG pathway enrichment and GO analysis， and the protein-protein interaction （PPI） network was constructed to analyze the interaction between DEGs expressed proteins. Results：A total of 29 DEGs were screened from three data sets. KEGG pathway enrichment analysis and GO analysis showed that the inflammatory immune response was activated after H. pylori infection. PPI analysis showed that the activities of TLR4， ICAM1， CCL4， TLR5， CCL19 and CR2 were enhanced after H. pylori infection. Conclusion：The inflammatory response of H. pylori infection is active， and TLR4， ICAM1， CCL4， TLR5， CCL19， and CR2 genes can be used as potential biomarkers for the treatment of H. pylori infection.

Analysis of Short-Term Prognostic Factors in Alcoholic Liver Disease-Related Acute-on-Chronic Liver Failure

Yao Qiuyan, Wu Zesheng, Shi Rognjie, Yang Liwei

2023, 8(2): 36-41.

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Objective：To analyze the influencing factors of short-term prognosis of patients with alcoholic liver disease （ALD） related acute-on-chronic liver failure （ACLF）， and to establish a short-term prognosis evaluation model. Methods： The baseline clinical data of 96 patients with ALD-ACLF were retrospectively analyzed， and they were divided into survival group and death group according to the 90-day survival. Logistic regression analysis was used to screen the independent influencing factors of 90-day prognosis， and a prognostic evaluation model was established. Results： Among the 96 patients with ALD-ACLF， 88 patients were male. The main basis of liver disease was cirrhosis， and the main complications were ascites and infection. Logistic regression analysis showed that total bilirubin （TBIL）， prothrombin time （PT）， neutrophil-lymphocyte ratio （NLR） and co-infection were independent prognostic factors of patients with ALD-ACLF at 90 days. The prognostic evaluation model was established， which was 0.013×CTBIL+0.085×CNLR+0.575×CPT. The optimal cut-off point of the model was 16.34， the specificity was 77.55%， the sensitivity was 87.23%， and the area under the curve was 0.913. Conclusion： The prediction model established in this study has certain predictive value for short-term prognosis of patients with ALD-ACLF.

The Expression of Serum Progranulin in Patients with Graves Disease and Its Correlation with IL-17 and IL-10

Song Qiuling, Dai Jiao

2023, 8(2): 42-45.

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Objective： To investigate the expression of serum progranulin（PGRN） in patients with Graves disease（GD） and its correlation with interleukin-17（IL-17） and interleukin-10（IL-10）. Methods： The serum PGRN， IL-17 and IL-10 levels of GD patients and healthy subjects were measured by enzyme-linked immunosorbent assay， and the correlation between PGRN and IL-17 and IL-10 was analyzed by Spearman correlation analysis. Results： Compared with the healthy subjects， the serum levels of PGRN and IL-17 in GD patients were significantly higher， while the level of IL-10 was significantly lower， with statistically significance （P<0.01）. The results of Spearman correlation analysis showed that serum PGRN levels in GD patients were significantly positively correlated with IL-17 levels， and negatively correlated with IL-10 levels. Compared with before treatment， the levels of PGRN and IL-17 in serum of GD patients treated with 131I decreased significantly， while the level of IL-10 increased significantly， with statistically significance（P<0.01）. Conclusion： The level of serum PGRN in GD patients is elevated， and it is significantly correlated with the levels of IL-17 and IL-10， suggesting that it may be involved in the occurrence and development of the disease.

A Comparative Study of the Clinical Efficacy of Endoscopic Thyroidectomy via the Thoracic-Breast Approach and Open Thyroidectomy

Cheng Wei, Shi Haibin, Cun Xiangnong, Ai Kewei, Fan Jiaju, Yang Jiwu

2023, 8(2): 46-50.

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Objective： To compare the clinical efficacy of endoscopic thyroidectomy via the thoracic-breast approach and open thyroidectomy in the treatment of benign and malignant thyroid diseases. Methods：From April 2011 to November 2021， the clinical data of patients who underwent endoscopic thyroidectomy and open thyroidectomy in Department of the Second General Surgery of the First Affiliated Hospital of Dali University were retrospectively analyzed. According to the operation mode， the patients were divided into endoscopic group and open group. The operation time， intraoperative bleeding， hospital stay， postoperative complication rate， postoperative pain 24 hours and incision satisfaction 1 month after operation were compared between the two groups. Results： There was no significant difference between the two groups in terms of operation time， hospital stay and postoperative complication rate（P >0.05）. Compared with the open group， the patients in the endoscopic group had significantly reduced intraoperative bleeding， significantly reduced postoperative pain， and significantly improved their satisfaction with the incision， with a statistically significant difference（P<0.05）. Conclusion： Compared with the traditional open surgery， endoscopic thyroidectomy via the thoracic-breast approach has the advantages of less bleeding， less postoperative pain， and good cosmetic effect， which is worth promoting in the surgical treatment of thyroid nodules.

Study of the Distribution of High-Risk Human Papilloma Virus Subtypes in Liangshan Region， Sichuan Province

Chen Huaqiu, Chen Xingyou, Wang Guangming

2023, 8(2): 51-55.

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Objective： To understand the characteristics of high-risk human papillomavirus （HR-HPV） infection among women in Liangshan Region， Sichuan Province， and to provide data support for the development and application of HPV vaccine in this area.Methods： The PCR fluorescence probe method was used to detect HR-HPV in 7 445 women who visited the physical examination center of Xichang People's Hospital and the outpatient department of obstetrics and gynecology from November 14，2018 to December 30，2020， and the detection results were statistically analyzed. Results：Among the 7 445 samples， 1 027 were HR-HPV positive， with a positive rate of 13.79%， and all 15 HR-HPV subtypes were detected. Among them， 818 cases （79.65%） were single infection， and 209 cases （20.35%） were multiple infection. The top three subtypes of HR-HPV infection were HPV 52， 16 and 53. In all age groups， the positive rate of subjects aged ≥ 61 years was the highest （22.39%）， followed by subjects aged 21-30 years （15.86%）. Conclusion： The distribution of HR-HPV subtype infection in Liangshan Region， Sichuan Province has certain regional characteristics， and the positive rate is the highest among people over 61 years old. Therefore， it is necessary to strengthen the publicity of HR-HPV screening among the suitable age population.

Changes of MiR-1281 and MiR-122 in Peripheral Blood of DR Patients and Their Relationship with the Severity of Retinopathy

Tian Tian, Yan Jing, Dai Jiao

2023, 8(2): 56-61.

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Objective： To investigate the changes and clinical significance of miR-1281 and miR-122 in peripheral blood of patients with diabetic retinopathy （DR）. Methods： 55 patients with type 2 diabetes without retinopathy （NDR group）， 42 patients with non-proliferative DR （NPDR group）， 30 patients with proliferative DR （PDR group）， and 40 healthy people （control group） were selected as the study subjects. The relative expression of miR-122 and miR-1281 in serum was determined by real-time fluorescence quantitative polymerase chain reaction. Results： The serum miR-122 level in NPDR group and PDR group was significantly higher than that in control group and NDR group， and that in PDR group was significantly higher than that in NPDR group （P<0.05）. The level of miR-1281 in NDR group， NPDR group and PDR group was significantly higher than that in the control group， and the expression level of miR-1281 in NDR group， NPDR group and PDR group was significantly higher than that in the control group（P<0.05）. Serum miR-122 and miR-1281 were significantly and positively correlated with the course of disease， glycosylated hemoglobin and HOMA-IR index. The area under the curve of miR-122 and miR-1281 for differentiating proliferative DR was 0.81 and 0.86， respectively. Conclusion： The expression of serum miR-122 and miR-1281 increased in DR patients， which can be used as an index to evaluate the severity of DR.

Expression and Prognostic Significance of Ki-67， Bcl-6， Bcl-2 and TEFM in Diffuse Large B Cell Lymphoma

Sun Xiaomei, Mei Wen, He Bing, Luo Zhonggui, Sun Ruli, Xiong Wei, Zhao Yi

2023, 8(2): 62-69.

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Objective： To explore the expression of Ki-67， Bcl-6， Bcl-2 and TEFM in diffuse large B cell lymphoma （DLBCL） and their relationship with the clinical characteristics and prognosis of patients. Methods： The expressions of Ki-67， Bcl-6， Bcl-2 and TEFM in 41 cases of DLBCL tissues and 42 cases of reactive proliferative lymphoid tissues were compared. The correlation between the expression levels of Ki-67， Bcl-6， Bcl-2 and TEFM and the clinicopathology of DLBCL was analyzed， and the prognostic factor of DLBCL patients were screened. Results： The expression level of Ki-67， Bcl-2 and Bcl-6 in DLBCL tissue were significantly higher than those in non-germinal central reactive proliferative lymphoid tissue，and the difference was statistically significant（P<0.05）. Ki-67 and TEFM protein expression levels were correlated with the primary site （P<0.05）. The 3-year survival rate of DLBCL patients was related to the age of patients， and the 3-year survival rate of patients aged ≥60 years was only 28.57%. Conclusion： Ki-67， Bcl-6 and Bcl-2 are highly expressed in DLBCL tissues. TEFM may have a synergistic effect on Ki-67， Bcl-6 and Bcl-2 in the progress of DLBCL. Ki-67， Bcl-6 and Bcl-2 can be used as important indicators of poor prognosis of DLBCL.

The Value of Quantitative CT in Bone Mineral Density Measurement of Patients with Chronic Kidney Disease

Xia Wangxu, He Yongfang, Chen Xiaobo, Huang Chuanfen

2023, 8(2): 70-73.

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Objective： To investigate the value of quantitative CT in measuring bone mineral density in patients with chronic kidney disease（CKD）. Methods： From January 2019 to December 2021， CKD patients（case group） diagnosed in Chongqing Changshou District People's Hospital and healthy volunteers（control group） who underwent bone mineral density examination at the same time were selected as the research objects. The bone mineral density was measured by dual-energy X-ray absorptimetry and quantitative CT. The research objects were divided into normal bone mass subgroup， low bone mass subgroup and osteoporosis subgroup according to the T value. According to the stage of CKD patients， the case group were divided into CKD stage 1-2 subgroup， CKD stage 3 subgroup， CKD stage 4 subgroup and CKD stage 5 subgroup. The bone mineral density of each group was compared， and the diagnostic value of quantitative CT for osteoporosis in CKD patients was evaluated by the receiver operator characteristic curve（ROC curve）. Results： The overall differences of bone mineral density in different bone mass subgroups were statistically significant， and the differences between groups were also statistically significant（P<0.05）. There was a statistically significant difference in the overall bone mineral density between the control group and different CKD subgroups， and there was a statistically significant difference in the bone mineral density between CKD stage 4 subgroup， CKD stage 5 subgroup and the control group （P<0.05）. The ROC curve results showed that the sensitivity and specificity of quantitative CT in detectingbone mineral density for osteoporosis in CKD patients were 80.99% and 83.10%， respectively， with good diagnostic efficacy. Conclusion： Quantitative CT can be used to measure bone mineral density in CKD patients， which has high diagnostic value for osteoporosis in CKD patients.

Comparison of Three Detection Methods for Urinary Sediment

Dong Liwen, Wang Caihong, Zou Tuanbiao

2023, 8(2): 74-77.

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Objective： To investigate the detection of urine sediment in patients by three methods. Methods：Urine analyzer， dry chemistry analyzer and microscope were used to analyze the visible components in 1 000 urine samples from a secondary first-class hospital in Kunming， and the test results and diagnostic coincidence rate were compared. Results： Among 1 000 urine samples， the detection rate of microscopic examination for white blood cells， red blood cells， bacteria， crystals， epithelial cells and fungi was high， the detection rate of urine analyzer for casts was high， and the dry chemistry analyzer could detect nitrite and protein that could not be detected by the above two methods. The combined test of red blood cells had the highest diagnostic coincidence rate， while the combined test of crystals， casts and epithelial cells had the lowest diagnostic coincidence rate. Conclusion： Both urine analyzer and microscopic examination are effective methods for urine examination， which have important diagnostic value. Dry chemistry analyzer can make up for the shortcomings of the above two methods to some extent. The three methods should be combined in clinical urine test to provide more reliable test results for clinical use.

Epidemiological Characteristics and Trend Prediction of Influenza in Yuxi City from 2010 to 2019

Ni Zhaolin, Zuo Shunwu, Liu Hongyan, Ai Zhiqiong, Nong Luming, Zhao Qiufang, Wu Qiang

2023, 8(2): 78-83.

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Objective： To analyze the epidemicological characteristics of influenza in Yuxi city from 2010 to 2019， and to establish an autoregressive integrated moving average（ARIMA） model to predict the trend of influenza incidence. Methods： The data of influenza was analyzed by descriptive epidemiological methods， and the ARIMA model was established by SPSS 25.0 software. Results： A total of 9 925 influenza-like cases were detected in Yuxi influenza network laboratory， and 2 849 nucleic acid positive cases were detected， with a positive rate of 28.71%. The nucleic acid positive cases showed an upward trend year by year in general， with an average increment speed of 2.01%. Among influenza positive cases， the ratio of male to female was 1.25∶1， and children aged 5-14 accounted for the highest proportion （40.86%）. Influenza had a strong seasonality（concentration was 0.60）. The dominant influenza virus strains changed year by year. In 2010 and 2018， A/H1N1 pdm was dominant； in 2011 and 2012， B/Victoria was dominant； in 2013 and 2016， B/Yamagata was dominant； and in 2015 and 2017， A/H3N2 was dominant； in 2014， B/Yamagata and A/H1N1 pdm dominated， and in 2019， B/Victoria and A/H3N2 dominated. The best prediction model was ARIMA（1，0，0）（0，1，1）12， whose standardized BIC value was 5.873， Ljung-Box Q was 21.258， P was 0.169. The actual values of the prediction results were within the 95% confidence interval（95%CI） of the predicted value， and the average relative error was 10.14%. The model was used to predict a decreasing trend of influenza incidence from January to June in 2020. Conclusion： The influenza in Xuyi city has obvious seasonality and the nucleic acid positive cases show an upward trend year by year in general， and the dominant influenza strains change year by year. ARIMA（1，0，0）（0，1，1）12 model can effectively predict the trend of short-term influenza in Yuxi city.

Application of Drawing the Vascular Site Map under B-Ultrasound in Rope Ladder Puncture

Miao Zhifei, Huang Zhaodan, Chen Yanqing, Chang Yanping

2023, 8(2): 84-87.

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Objective： To explore the effect of drawing the vascular site map by cooperation between doctors and nurses under B-ultrasound positioning in planned rope ladder puncture of hemodialysis patients. Methods： 120 patients with maintenance hemodialysis in the Blood Purification Center of the Third People's Hospital of Yunnan Province were selected as the study subjects. According to the method of rope ladder puncture， the patients were divided into control group and observation group. The control group was given routine rope ladder puncture， and the observation group was carried out planned rope ladder puncture with doctors and nurses jointly drawing the vascular site map under the positioning of B-ultrasound. The success rate of one puncture， the implementation rate of rope ladder puncture， patient satisfaction and the complication rate of arteriovenous fistula were compared between the two groups. Results： The success rate of one puncture， the implementation rate of rope ladder puncture and patient satisfaction in the observation group were higher than those in the control group， and the difference was statistically significant（P<0.05）. The complication rate of arteriovenous fistula in the observation group was significantly lower than that in the control group （P<0.05）. Conclusion： The cooperation of doctors and nurses in drawing the vascular site map under the positioning of B-ultrasound can enable nurses to better implement the rope ladder puncture technology， improve the success rate of one puncture and patient satisfaction， reduce the occurrence of arteriovenous internal fistula complications， and extend the service life of internal fistula， which is worth promoting in clinical work.

Application Analysis of Individualized Follow-Up Guidance Based on Network Platform in Patients with Basal Insulin

Zhu Xiaoli, Shi Tingting, Zhang Likun, Wei Yanni, Li Ting, Zhang Xiaoling

2023, 8(2): 88-92.

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Objective： To analyze the effect and compliance of individualized follow-up guidance based on the network platform on blood glucose control in patients using basal insulin for the first time. Methods：From January 2018 to May 2019， 539 patients who first used basal insulin in the First Affiliated Hospital of Dali University were given individualized follow-up guidance on the network platform. The general information and blood glucose monitoring data at various stages of the follow-up patients were retrospectively analyzed， and multiple linear regression analysis was performed on many factors. Results： During the follow-up period， the rate of fasting blood glucose up to standard was （69.73±3.25）%， and the rate of up to 2 h postprandial blood glucose was （74.63±1.49）%. Multiple linear regression analysis found that there was a correlation between the initial fasting blood glucose and the initial insulin dose（P<0.05）， and there was a correlation between the blood glucose at 2 h after meals when included in the study group and the course of disease， age and body mass index（P<0.05）. Conclusion： The individualized follow-up guidance based on the network platform can improve the glycemic control effect of the first-time insulin users and improve the patient's treatment compliance with insulin medication.

Application of KAP Health Education in Nursing Care of Elderly Patients with Hypertension

Ma Yunchu, Bi Jing, Yang Jianyun, Cui Dehua

2023, 8(2): 93-96.

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Objective： To explore the effect of health education based on KAP model in the nursing care of elderly patients with hypertension. Methods： 83 elderly hypertensive patients admitted to the Department of Cardiovasology of Qujing Second People's Hospital from July 2018 to June 2019 were selected as the research objects， and randomly divided into the observation group and the control group. The control group was given routine health education， while the observation group was given health education based on KAP model. The nursing effects of the two groups were compared. Results： After nursing， the levels of systolic blood pressure and diastolic blood pressure in the observation group were significantly lower than those in the control group， and the scores of each dimension and total score of the self-management behavior scale and the scores of each dimension of the quality of life scale were higher than those in the control group， with statistical significance（P<0.01）. The nursing satisfaction rate of the observation group was significantly higher than that of the control group， and the difference was statistically significant（P<0.01）. Conclusion： The application of health education based on KAP model in the nursing of elderly patients with hypertension has a good effect， and it is worth popularizing.

Analysis of the Application Effect of Video Assessment in Nursing Clinical Operation Skills Training

Liu Li, Qian Liyun, Jiang Xian, Shi Aiyun, Wang Liqian, Huang Hongli

2023, 8(2): 97-100.

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Objective： To analyze the application effect of video assessment in nursing clinical operation skills training. Methods： The nurses participating in the clinical operation skill assessment were randomly divided into the control group and the experimental group， with 100 in each group. The control group used the traditional examination method， and the experimental group used the video examination method. The clinical operation skill examination results and the satisfaction of nurses and invigilators in the two groups were compared. Results： The test scores of nurses in the experimental group were higher than those in the control group， and the difference was statistically significant（P<0.05）. The results of the survey showed that the satisfaction scores of the nurses and invigilators in the experimental group for 11 survey items were higher than those in the control group， and the difference in satisfaction scores for most of the survey items was statistically significant（P<0.05）. Conclusion： Video assessment method can effectively improve nurses' clinical operation skills， improve the assessment satisfaction， and improve the teaching effect， and it is worthy of promotion in nursing clinical operation skills training.

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