〔Abstract〕Objective：To predict the mechanism of DangGuiBuXue decoction in treating anemia by using network pharmacology technique. Methods: TDT and TCMSP databases were used to screen the chemical composition of DangGuiBuXue decoction; OMIM database screened the targets of anemia; PPI database established the interaction targets between the drug and the disease. Cytoscape software was applied to construct the "compound-target-disease" complex network diagram. Targets of DangGuiBuXue decoction were selected, which played an important role in treating anemia by using the network topological parameters such as Degree, Betweenness Centrality, Closeness Centrality. In the "component-target-disease" network of DangGuiBuXue decoction, the targets which achieved the standard were enriched by the DAVID database. Results: 15 targets correlated with anemia were screened. DangGuiBuXue decoction was useful for treating anemia via regulating cell cycle arrest and eleven pathways such as Cell cycle, P53 signaling pathway, etc. Conclusion: The mechanism of DangGuiBuXue decoction in the treatment of anemia has been predicted by network pharmacology, which can provide the theoretical basis for its clinical application.
〔Abstract〕Objective：To predict the mechanism of ephedrae herba-cinnamomi ramulus herbal pair in treating hydropsy by using network pharmacology technique. Methods: TDT and TCMSP databases were used to screen the chemical composition of ephedrae herba and cinnamomi ramulus; OMIM database screened the targets of hydropsy. A "compound-target-disease" complex network diagram was constructed with Cytoscape software. In the "component-target-disease" network of ephedrae herba and cinnamomi ramulus, the gene function and KEGG path of the selected targets were analyzed. Results: 10 protein targets effective in treating hydropsy had been screened. It was found that their treating effect was achieved mainly through regulating VEGF signaling pathway and vascular endothelial cell migration. PI3K-Akt signaling pathway, TNF signaling pathway, Ras signaling pathway and MAPK signaling pathway also played significant roles. Conclusion: The mechanism of ephedrae herba and cinnamomi ramulus in the treatment of hydropsy has been predicted by network pharmacology, which can provide the theoretical basis for its clinical application.
〔Abstract〕Objective: To investigate the therapeutic effect of Ganlong capsule on rat liver fibrosis induced by pig serum. Methods: After the rat liver fibrosis model was induced by porcine serum, rats with liver fibrosis were treated with different concentrations of Ganlong capsule. The liver, spleen, thymus were weighed and their index were calculated. The index of liver function was detected with the kit, including γ-glutamyl transpeptidase and malondialdehyde. Enzyme-linked immunosorbent assay（ELISA）was used to detect the indexes of liver fibrosis including Laminin, Hyaluronic acid, PCⅢ, Ⅳ-C, Type collagen and transforming growth factorβ1. Results: Ganlong capsule had reduced the content of LN, PCⅢ and Ⅳ-C in fibrosis serum of rats, decreased the content of γ-GT and HA, and increased the content of COL-Ⅰ in liver tissue. Conclusion: Ganlong capsule has certain therapeutic effects on liver fibrosis in rats.
〔Abstract〕Objective: To study the inhibitory effects of three ethanol extracts of Blaps rynchopetera Fairmaire（A, B, C）on proliferation of human embryonic lung diploid fibroblast, and to compare the inhibition effects of the three extracts. Methods: Human embryonic lung diploid fibroblast were incubated with extract A, B and C of 3.9~1 000.0 μg/mL in vitro. MTT was used to detect the effects of three ethanol extracts of Blaps rynchopetera Fairmaire on proliferation of cells. Result: High concentrations of three ethanol extracts of Blaps rynchopetera Fairmaire significantly inhibited the growth of human embryonic lung diploid fibroblast and showed a time-dose-effect dependence. At the concentration of 1 000.0 μg/mL and after 72 h, the inhibitory rates of A, B and C on the cells reached the highest, which were 49.87%, 50.13%, and 42.34%, respectively. There were no significant differences in the proliferation activity of A, B and C. Conclusion: The ethanol extracts of Blaps rynchopetera Fairmaire can significantly inhibit proliferation of human embryonic lung diploid fibroblast, and the inhibitory effects of A, B and C on the cells were not significant.
〔Abstract〕Objective: After 28 foreign species of AM fungi were inoculated in sterilized soil, the effects of the AM on mycorrhizal colonization and the diosgenin content of Paris polyphylla var. yunnanensis were observed by inoculation test in pot at room temperature. Methods: Trypan blue staining was used to test AM colonization, and HPLC was applied for analyzing diosgenin content. Results: The good symbiotic relationship between Paris polyphylla var. yunnanensis and AM fungi species was observed, and AM fungi species enhanced the colonization. Most AM fungi species did not influence the diosgenin content（0.978 2~10.745 3 mg/g）compared with the control group（CK）. However, Svi fungi significantly increased the diosgenin content（41.404 5 mg/g）. Conclusion: There are symbiotic relationships between arbuscular mycorrhizal（AM）fungi and Paris polyphylla var. yunnanensis. AM fungi species can enhance the colonization, and Svi fungi species can greatly increase the diosgenin content.
〔Abstract〕Objective: To perform pharmacognostic studies on Phlomis forrestii Diels. Methods: We identified the sources and determined the shape properties with the help of microscopic and physiochemical ways. Results: Cangshancaosu is the dry complete grass or the whole plant of Phlomis forrestii Diels. Its crude drug identification was described in details. Conclusion: The experimental results can provide the theoretical basis for further development, utilization, and identification of Phlomis forrestii Diels.
〔Abstract〕Objective: To elaborate the medical theoretical system of Bai nationality and its particularity. Methods: By the use of literature research and field investigation, the theoretical system and characteristics of the Bai medicine was summarized and concluded. Results: Bai medicine was created and accumulated in the development of Bai people's history, and a relevant theoretical system has been formed in the long term of medical practice. Conclusion: Though Bai medicine has been profoundly influenced by the traditional Chinese medicine, as it is rooted deeply in the soil of the Bai culture of Dali area, Bai medicine shows its peculiarity.
〔Abstract〕Objective: To explore interesting methods in pharmacology teaching. Methods: Based on 30-year teaching experience as for pharmacology, this paper tries to analyze and explore how to improve the methods in pharmacology teaching. Results: Because the content of pharmacology is rich and complicated, with complex theories involved in it, it seems that the course is a little bit tedious and students often feel it difficult to learn. It has proved that interesting and vivid teaching methods have been very useful and helpful to increase students' learning interest. Conclusion: It is easy for students to accept such kind of easy and understandable teaching ways.
〔Abstract〕Objective: To explore new measures for the reform of hospital pharmacy practice under the new situation of medical reform, and to provide a reference for improving the comprehensive skill level of pharmacy graduates. Methods: 44 pharmacy interns in the Sixth Affiliated Hospital of Dali University in 2017 were set as the experimental group, and 41 pharmacy interns from 2014 to 2016 were set as the control group. The control group was taught with the traditional teaching method, while the experimental group adopted the teaching reform measures. The effects of teaching reform measures were evaluated from three aspects: questionnaires, student assessment results, and teaching quality evaluation results. Results: Teaching reform measures enhanced students' self- confidence and role transformation consciousness, effectively motivated students' enthusiasm for learning and independent thinking ability. The scores of the experimental group students were higher than those of the control group, and the difference was statistically significant（P<0.05）. The quality of teaching in our department was greatly improved（P<0.05）. Conclusion: Our teaching reform measures can effectively improve the comprehensive ability of pharmacy students and adapt to the new situation in which pharmacists' roles have been transformed. It is worthy of reference and needs to be improved.
〔Abstract〕Objective：To observe the curative effect of treating tuberculous cervical lymphadenopathy with ultrasound-mediated targeted drug delivery. Methods：120 patients with cervical lymph node tuberculosis were randomly divided into three groups with 40 in each group: the treatment group of ultrasound medicines transdermal, the local injection group and the control group. Three groups were all treated with conventional anti-tuberculosis therapy. The control group was treated with routine anti-tuberculosis chemotherapy and without partial treatment. The treatment group was given percutaneous thrombin injection of Isoniazid and Amikacin under ultrasound guidance. The local injection group was injected with Isoniazid and Amikacin. Besides comparing curative effects of the three groups, we observed the size of lymph node of the experimental group in different time periods. Results: The overall curative effect of the experimental group was higher than that of the local injection group and the control group, and there was a significant statistic difference（P<0.05）; the effective rate of the treatment group reached the highest within 28 days, and there were no significant differences after 21-day and 28-day treatment（P >0.05）. Conclusions：It is a safe and effective way to treat lymph node tuberculosis with ultrasonic penetration, and the best advisable treatment course is from 21 to 28 days.
〔Abstrac〕Objective: To compare the effect of pancreaticojejunostomy internal and external drainage on the postoperative complications during pancreato duodenectomy. Methods: We collected and comparatively analyzed the clinical data of 37 patients who underwent internal drainage of pancreatic intestinal anastomosis and 36 patients who underwent external drainage of pancreatic intestinal anastomosis in the First Affiliated Hospital of Dali University from January 2000 to December 2017. Results: The postoperative hospital stay and the incidence of gastric retention were lower in the internal drainage group than in the external drainage group, and the difference was statistically significant（P<0.05）. There was no significant difference in operative time, intraoperative blood loss, postoperative pancreatic fistula, abdominal bleeding and abdominal infection rate between the two groups（P > 0.05）. No deaths occurred during the perioperative period in both groups. Conclusion: Intra-abdominal anastomosis drainage can reduce postoperative hospital stay and gastric retention, which is more suitable for clinical promotion.
〔Abstract〕Objective: To investigate the diagnosis and treatment of spontaneous abdominal wall hematoma by reviewing clinical cases. Methods: The clinical data of 13 patients with spontaneous abdominal hematoma admitted to the First Affiliated Hospital of Dali University from 2010 to 2017 were analyzed. Results: There were 5 cases with conservative treatment, 7 cases with hematoma removal, 1 case with misdiagnosis of appendicitis, and 13 cases were cured. Conclusion: A comprehensive and detailed medical history is required. Early diagnosis and early treatment can be done in combination with relevant imaging examinations to avoid delay of illness.
〔Abstract〕Objective: To promote rational use of drugs and ensure the safety of drug use in children according to JCI standard. Methods: A retrospective analysis has been conducted on the results of intervening 1 249 unreasonable prescriptions in Pediatric Wards in 2017. Results: There were 1 249 unreasonable prescriptions in total, of which 506（40.51%）had problems concerning inappropriate usage and dosage, 391（31.31%）had problems of improper or undear writing of drug dosage or specifications, 305 （24.42%）didn't show standardized or clear usage of drugs, 33（2.64%）repeated the same drugs, 12（0.96%）had problems of compatibility taboo or undesirable interaction, and 2（0.16%）had problems of improper combination of drugs. Conclusions: Clinical physicians should regulate the formulation of prescription and doctor's orders, and pharmacists should strengthen the work of prescription checking in order to promote safe, effective, economic, reasonable pharmacy prescriptions of hospitalized children.
〔Abstract〕Objective: To observe the clinical effect of new supramolecular salicylic acid combined with facial care in the treatment of acne vulgaris. Methods: A total of 72 patients with acne vulgaris were randomly divided into two groups. The control group consisted of 34 patients who were treated with new supramolecular salicylic acid for once every two week; there were 38 patients in the treatment group who were treated with the combination of the new supramolecular salicylic acid and facial care. After a week of the treatment of salicylic acid, the latter was infused with homemade solution with the help of ultrasonic wave for once or twice a week. Both of the two groups were treated with salicylic acid for one course. And then a month later, the curative efficacy in the two groups were compared. Results: The cure rate in the treatment group was significantly higher than that of the control group，and the incidence of adverse reactions in the treatment group was significantly lower than that in the control group. There were statistically significant differences between the two groups（P＜0.05）. Conclusion: The combination of new supramolecular salicylic acid and facial care in the treatment of acne vulgaris is much more effective than the method of using salicylic acid alone, with the ability to improve skin barrier function and reduce adverse effects. It is worthy of clinical application.
〔Abstract〕Objectives: To survey the prevalence of swine hepatitis E virus in pigs in Dali, and provide a theoretical basis for preventing and controlling swine hepatitis e virus. Methods: Enzyme-linked immunosorbent assay（ELISA）was used to detect HEV- IgG and HEV-IgM in 510 serum samples, the immunohistochemical staining method was used to detect HEV antigen in 152 liver samples, and the liver samples were histopathologically sectioned and stained by hematoxylin and eosin for microscopic observations. Results: ① The positive rates of HAV-IgG and HEV-IgM were 17.5%（89/510）and 6.3%（32/510）, respectively, none of the samples was both HEV- IgG and HEV- IgM positive, and the positive rate of anti- HEV was 23.7%（121/510）. ② Histopathological observations showed different degrees of pathological changes could be found in 68.4%（104/152）liver tissue samples. ③ The results of immunohistochemical stains indicated that the HEV antigen positive rate was 63.2%. Conclusion: There is a high prevalence of HEV in swine herds in Dali, and relevant government departments should attach importance to it and take corresponding measures.
〔Abstract〕Objective: To investigate and evaluate the health hazards of mobile phone use in a medical college, and to provide reference for reducing health risks. Methods: A stratified random sampling survey was conducted by distributing self- designed "Perception Questionnaire" which includes the basic information about students, the cell phone functions used continuously, and subjective perceptions of health hazards after a continued usage. The SPSS 18.0 software was used to analyze the reliability and validity of the scale with Cronbach's alpha coefficient and factor analysis. Results: It indicated that the scale had good internal consistency and validity, with the Cronbach's α coefficient, cumulative explained rate, communality and max factor loading greater than 0.90, 70%, 0.50 and 0.55, respectively. More than 80% of respondents continue to use such mobile phone features as "social networking", "watching video", "game and entertainment" and "reading and learning" for a long time（≥1 h）. The health risks for neck/shoulder and eye were moderate, while risks for ear and nervous system were mild after a long time of mobile phone use. Conclusion: It is essentials for students to use mobile phones reasonably, trying to avoid mobile phone dependence, and develop good habits of mobile phone use, which is conducive to reducing heath risks and hazards.
〔Abstract〕Objective: To investigate knowledge, attitude and practice of laboratory biosafety among students of Dali University, and provide basic data for laboratory biosafety education. Methods: Using stratified cluster sampling, the study was conducted among students of Dali University, and a self-administered KAP questionnaire was used to carry out the survey. Results：1 050 questionnaires were distributed and 1 042 were valid. Students' KAP of laboratory biosafety were evaluated，and the passing rates of KAP were 29.8%, 93.5% and 89.9%, respectively. There was a positive correlation between K-A-P. The passing rate of laboratory biosafety knowledge was related to students' academic degree, grade and college; the passing rates of laboratory biosafety attitude and practice were related to students' gender, academic degree, college and specialty. Conclusion：The students' knowledge of laboratory biosafety needs to be reinforced. It is necessary to strengthen laboratory biosafety education by setting up laboratory biosafety courses and conducting regular publicity and training for junior students in order to ensure the university's laboratory biosafety and build a safe campus.
〔Abstract〕Objective: To understand the status of job burnout, coping style and general well-being of nurses in ethnic minority areas, and to provide the basis for the prevention and reduction of job burnout among minority districts nurses. Methods: 592 nurses in Dali prefecture were surveyed by the use of Maslach Burnout Inventory, Simplified Coping Style Questionnaire and General Well-being Schedule. Results: ① Based on the standards of critical value and component number, the degree of nurse job burnout was lower in Dali than in Hangzhou and Xinjiang. ② There were differences in the depersonalization among different ethnic groups. ③ Emotional exhaustion was negatively correlated with general well-being, and positively correlated with negative coping; depersonalization was negatively correlated with general well-being and positive coping, and positively correlated with negative coping; personal accomplishment was positively correlated with positive coping and negatively related to negative coping. Conclusion: It is an effective way to improve the general well-being of nurses in ethnic minority areas by reducing the emotional exhaustion and depersonalization, and improving the personal accomplishments.
〔Abstract〕Objective: To explore the effect of individualized psychological nursing on patients undergoing Flexible ureteroscope holmium laser lithotripsy. Methods: 68 patients treated with flexible ureteroscope holmium laser lithotripsy from June 2016 to January 2018 in the First Affiliated Hospital of Dali University were selected and randomly divided into two groups: nursing intervention group （experimental group）and conventional nursing group（control group）, with 34 cases in each group. Then we investigated patients' satisfaction and the changes of heart rate before and after nursing in the two groups. Results: After receiving individualized psychological nursing, the satisfaction degree over nursing quality and the decrease of heart rate in the experimental group were significantly higher than those in the control group, and the difference was statistically significant （P＜0.05）. Conclusion: For patients who are treated with flexible ureteroscope holmium laser lithotripsy, fully individualized psychological nursing before surgery can arouse the patients' enthusiasm and subjective initiative, improve their satisfaction with the quality of nursing, and stabilize their heart rate. It can help patients better cooperate with surgical treatment and recover quickly.
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