Table of Content

15 April 2015, Volume 14 Issue 4

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Evaluation of Antimalarial Activity of the Ten Herbs from West Yunnan

Liu Ziqi, Xiao Chaojiang, Zhang Xiaodong, Xu Wei, Peng Junlin, Jiang Bei

2015, 14(4):  1-4. 

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Objective: To study the antimalarial activity of ten herbs collected from west Yunnan for the development of new
generation of antimalarial drugs. Methods: After being milled, the plant materials were extracted three times under reflux with 75%
ethanol and water successively. The extracts were completely dried using lyophilization, and the resulting samples were tested for the antimalarial activity by β-hematin inhibition screening assay. The activities were evaluated by IC50 values. Results: Four of the ten testing plants, Metapanax delavayi, Primula denticulate, Rhododendron trichocladum, and Corylopsis himalayana, showed moderate activity to inhibit β-hematin formation with the IC50 over 1 388.9 μg/mL. Conclusion: The four plants deserved to be further studied for their potential antimalarial activity.

Effect of the Extracts from Geranium strictipes R. Knuth on the Intestinal Propulsion Function in Mice

Geng Ling, Li Hongwen, Chen Junya, Li Hui

2015, 14(4):  5-7. 

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Objective: To study the effect of the ethyl acetate extract from Geranium strictipes R. Knuth（EAEG）on the intestinal
propulsion function in mice. Methods: Normal mice and mice of intestinal function inhibited by atropine were given different doses
EAEG for continuous 4 days, finally the propulsion distance of carbon in intestinal was measured. Results: EAEG-L（50 mg/kg）,
EAEG-M（100 mg/kg）and EAEG-H（200 mg/kg）all could increase the propulsion rate in normal mice, even the figure is up to
75.93% in EAEG-H group; in addition, EAEG-L（50 mg/kg）could improve apparently intestinal carbon propulsion rate which is
43.28%. Compared with control group, the difference is statistically significant. Conclusion: The acceleration of EGEG on intestinal
movement in normal mice and mice intestinal-inhibited by atropine may be related with M receptor.

Antibacterial Activities of the Ethyl Acetate Part and the Water Part of Geranium Trictipes R. Kunth to
Actinomyces Viscosus in Vitro

Yang Lin, Wang Tao, Lan Hai

2015, 14(4):  8-11. 

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Objective: To investigate the effect of the ethyl acetate part and the water Part of Geranium strictipes R. Kunth on the
growth, acidogenicity, adhesion of Actinomyces viscosus （A.v）. Methods: Actinomyces viscosus was chosen as the experimental
bacteria. And then the effects of the ethyl acetate part and the water portion of G. strictipes on the growth（including the minimum
inhibitory concentration and the minimum bactericidal concentration）, acid generation and adhesion were assessed. Results: The MIC of the ethyl acetate part and the water portion of G. strictipes against A.v was 4.500 g/L ,9.000 g/L, the MBC of the ethyl acetate part and the water Part of G. strictipes against A.v was 9.000 g/L ,18.000 g/L; it was found that the ethyl acetate part and the water Part of G. strictipes can inhibit the acid generation and adhesion of the A.v effectively. Conclusion: The ethyl acetate part and the water Part of G. strictipes may be an effective anti-caries agent.

Effects of Ganlong Capsule and Periplaneta americana Skim Cream on Acute Colitis in Mice

Niu Chunli, Zeng Jing, Xia Mengkai, Peng Fang

2015, 14(4):  12-16. 

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Objective: To study the effect of Ganlong capsule and Periplaneta americana skim cream on acute colitis in mice and to
further observe the mechanism of action. Methods: The experiment uses the DSS modeling method to evaluate the effects of Ganlong capsule and P. americana skim cream on acute colitis through the MPO content and the observation of colon tissue TNF- α
immunohistochemical and detection of mice colon tissue of calculation of DAI. Results: Ganlong capsule and P. americana skim cream can reduce infiltration of inflammatory cytokines, reduce mucosal bleeding ulcer, glands disappeared, reduce the expression of TNF-α,the Ganlong capsule groups and P. americana skim cream groups can reduce the content of MPO in the colonic tissues（P < 0.05）.Conclusion: Ganlong capsule and P. americana skim cream have very good protection effect on acute colitis induced by DSS.

Experimental Investigation into the Effects of Xigui on the Function of Monocyte of sleep-deprived Mice

Li Qiaoyun, Yu Zhengyong, Wang Na, Li Wanyu, Du Yi Min, Liu Xiaobo

2015, 14(4):  17-20. 

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Objective: To study the effect of ethanol extracts of Xigui on model non- specific immune function of mice sleep
deprivation. Methods: Mice were randomly devided into blank control group, sleep deprivation group, positive control group, and high dose, medium dose and low dose Xigui group. Gavage normal saline was applied to the blank control group and the sleep deprivation group, Gavage ginseng drug 34.01 g/（kg·d）was applied to the positive control group, high, medium and low dose Xigui group were respectively conducted crude drug 80.88 g/（kg·d）, 40.44 g/（kg·d）, 20.22 g/（kg·d）for continuous dosing nine days. In the tenth day, the sleep deprivation group, the positive control group and high, medium, low dose Xigui group were conducted SD in plain water environment. During the period of SD, experimental drugs were given. After three days, the effect of the drugs on the non-specific immune function of SD mice was observed by the carbon clearance test. Results: The K value and α value of Xigui in high , medium,low dose group compared with the SD group are significantly increase（P＜0.05 or P＜0.01）. Conclusion: Xigui could improve the immune function of SD mice.

Micro-management of GCP Implementation in Drug Clinical Trials

Gu Xiangfu, Zhu Xiaoli, Wang Xuechang

2015, 14(4):  21-25. 

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Objective: To discuss the micro-management of GCP implementation in drug clinical trials. Methods: Literature of GCP
in clinical trials were reviewed, analyzed and concluded. Results: Some principles of GCP need specific and detailed study.
Conclusion: Micro-management of GCP implementation is more operational, scientific and reliable for drug clinical trials.

The Expression of HCY-2 in Normal Human Embryonic Trachea and Lung

Zhang Yuanyuan, Li Yaokang, Wang Guangming

2015, 14(4):  26-29. 

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Objective: To investigate the expression of homocysteine induced gene（HCY-2）in the normal human embryonic trachea
and lung and to reveal the effects of HCY-2 on the development of respiration system. Methods: The immunohistochemistry ABC
method was used to detect the HCY-2 expression in the normal human embryonic trachea and lung. Results: HCY-2 expressed during
the whole development of the human embryonic trachea and lung and mainly in the epitheliums of trachea and pulmonary bronchi and
glandular epitheliums. Conclusion: HCY-2 expression protein could be closely related to the development of trachea and lung.

Clinical Analysis of 6 Cases of Pulmonary Alveolar Proteinosis

Nie Fangang, Su Ping, Yang Yuxue, Yang Wenjie

2015, 14(4):  30-34. 

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Objective: To explore the clinical features, diagnosis and therapy of pulmonary alveolus proteinosis（PAP）and improve
the level of clinical diagnosis and treatment to reduce misdiagnosis and incorrect treatment. Methods: Retrospective analysis of the
clinical features and chest CT data of patients with PAP in Baoshan People's Hospital in the last 9 years was made, the experience of treatment was summarized and reasons for misdiagnosis and incorrect mistreatment were analyzed. Results: Clinical symptoms were mainly polypnea and coughing after activities. And the chest high- resolution CT（HRCT）showed bilateral diffuse ground- glass opacities in 6 cases, a geographic pattern in 5 cases, a crazy paving pattern in 3 cases and air bronchogram in 2 cases. The misdiagnosis rate was high only with chest CT: 3 cases were misdiagnosed as pneumonia, 1 case as pulmonary tuberculosis and 1 case as idiopathic pulmonary interstitial fibrosis, prior to admission. 6 patients were diagnosed by bronchoalveolar lavage fluid（BALF）and transbronchial lung biopsy（TBLB）. In addition, their symptoms were markedly relived with BALF. Conclusion: Clinical manifestations of PAP were not specific, so its diagnosis was difficult and misdiagnosis and incorrect treatment were very likely if only based on HRCT. PAP should be considered when patients had polypnea and chest HRCT showed a geographic pattern and crazy paving pattern after activities. BALF was the most effective treatment. Blind treatment should be avoided when the diagnosis was not definite.

Interventional Embolization Therapy in Aneurysmal Subarachnoid Hemorrhage:
Clinical analysis of 102 Cases

Wu Weihua, Wu Tinshu, Xie Chongwu, Duan Liqiong, Xie Qingli, Li Dan, Zhang Xing

2015, 14(4):  35-37. 

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Objective: To analyze the clinical curative effect, complications and the preventive measures of interventional embolization
in treatment of aneurysmal subarachnoid hemorrhage. Methods: One hundred and two patients of aneurysmal subarachnoid hemorrhage were treated with interventional embolization, data were retrospectively studied. Results: Of all the 102 cases,91 cases were cured,7 cases were symptom improved with different degrees of limb dysfunction, 3 cases were automatic discharge and 1 case was dead. Sixty cases were outpatient follow-up from 6 month to 3 years after operation,10 cases were re-hospitalized and angiographic followed-up. There were 5 cases of recurrence and no rebleeding observed. Conclusion: Interventional embolization was an effective therapy in treatment of aneurysmal subarachnoid hemorrhage.

Plasma Spread under H-UPPP Surgery Treatment of Severe Obstructive Sleep Apnea Hypopnea Syndrome

Liu Hui, Wu Yanchun, He Yonggang, Liu Xiaoyu, Wang Liqiong, Bi Hui

2015, 14(4):  38-40. 

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Objective: To study plasma assisting H-UPPP surgery under low temperature, and the therapeutic effect of RFCA of the
hypertrophy of the soft palate, uvula and palatopharyngeal muscle treating severe OSAHS. Methods: Sixty cases of severe OSAHS with blocking plane in oropharynx under PGS monitoring were retrospectively analyzed. The melt- cut function of EIC5874 blade were applied to H-UPPP surgery, the thick soft palate, uvula and palatopharyngeal muscle were conducted RFCA with EIC4855 blade.Results: Postoperative patient's oropharyngeal cavity around, before and after were enlarged, the pharyngeal cavity structure, and function were complete. PGS monitoring were conducted to the postoperative 6 and 12 months follow-up, AHI values dropped, LSaO2 had statistical significance (P<0.01). Patients with subjective symptoms were improved, effective rate reached 91.7%. Conclusion: The surgical treatment of OSAHS is varied. For patients with severe OSAHS with the blocking plane in the oropharynx, plasma assiting HUPPP surgery under low temperature is distinguished by less bleeding, small trauma, and with the pharyngeal cavity structure and function preserved.

Experience in the Endoscopic Treatment of Colon Polyps

Du Guiying, Tao Zhongyuan, Guo Shunhong, Zheng Qingmei

2015, 14(4):  41-43. 

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Objective: To investigate the clinical methods and effect of endoscopic treatment of colon polyps. Methods: In 8 024
cases of enteroscope examination, We diagnosed 1 637 polyps from 1 326 cases in were diagnosed in 1 637. We applied single and combined with endoscopic therapy polyp according to different size and morphous. Results: Total 1 637 colon polyps were endoscopic Treated. Four cases were basement carcinogenesis and performed surgical therapy. Nine cases were delayed hemorrhage and successfully re-endoscopic haemostasis. Two cases were perforation, and 1 case was repaired successfully with titanium clip,1 case was titanium clip patching failed and performed surgical therapy. The total complication rate was 0.82% . Conclusion: endoscopic treatment was an effective and safe therapy in treatment of colon polyps.

Experience of Clinical Application of Distal Radial Artery Perforator Nasopharyngeal Fossa Ascending
Retrograde Fascial Flap

Guan Zhijiang, Yang Xiangdong, Shi Shengjun

2015, 14(4):  44-46. 

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Objective: To explore the clinical effect of nasopharyngeal fossa distal radial artery perforator ascending retrograde fascial
flap for the treatment of soft-tissue defects of the hand. Methods: 5 cases with soft-tissue defects of the hand were investigated who
received the treatment of the distal radial artery perforator of nasopharyngeal fossa ascending reverse fascial flap from October 2010 to August 2013. Results: Flaps of 4 cases survived completely, partial necrosis of flap margin was found in 1 case but healed after dressing, functional recovery was well achieved, 3 cases felt well, 2 cases felt dysaesthesia, and the circulation, color and texture of flaps were normal and the appearance was good-looking. Conclusion: The distal radial artery perforator of nasopharyngeal fossa ascending retrograde fascial flap for repair of soft-tissue defects of the hand was a treatment with good long-term effect and a lower price（without sacrificing the radial artery）and with no more operation needed.

Clinical Observation of rhEGF Treating Serious Contusion Wound

Zhang Chen, Chai Shaoqiang, Ma Binbin

2015, 14(4):  47-49. 

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Objective: To observe the effect of recombinant human epidermal growth（rhEGF） factor on serious contusion wounds
and its adverse reactions. Methods: Fifty-six cases with one hundred and forty-eight wounds were divided into two groups. On the
base of routine treatment, rhEGF was added to treatment group and 0.05% iodophor disinfection solution was conducted to control
group. The healing time of the two groups was observed respectively. Results: The average healing time for shallow Ⅱ degree
contusion wounds was 9.56±1.74 days for treatment group and 12.75±3.18 days for the control. The average healing time for deep II degree contusion wounds was 18.16±2.67 days in treatment group compared with 21.26±3.83 days in the control. The healing time was significantly shorter（P < 0.05, 0.01）in rhEGF group than the control. The effective rate in rhEGF group was 90.72%. Conclusion:rhEGF can promote healing in Ⅱ degree contusion wounds, especially for deep Ⅱ degree wounds.

Clinical Analysis of 35 Cases of Children with Staphylococcal Scalded Skin Syndrome

Chen Qiao, Ding Chuangang

2015, 14(4):  50-52. 

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Objective: To analyze the clinical presentation and diagnostic analysis of staphylococcal scalded skin syndrome（SSSS）
in children admitted in a hospital in the past nine years. Methods: Retrospective analysis was conducted to the clinical data of SSSS
admitted from October 2004 to October 2013. Results: Among the 35 infants treated in our hospital over the past nine years, fourteen were male and twenty-one were female aged from 22 days to 14 years old. Twenty-two cases were from 0 to 6 years old（62.8%）,among which one case was newborn（2.8 %）, 3 cases were younger than 1 year old（8.6%）, 8 cases were 1 to 3 years old children（22.8%）, 10 cases were 3 to 6 years old children（28.6%）, and 1 cases were 6 to 14 years old（37.2%）. Conclusion: Early diagnosis of SSSS combined with timely, adequate, sensitive antibiotic therapy is the most crucial of the disease.

Clinical Analysis of 27 Cases of Preschool Children with Ocular Trauma in Dali Region

Yu Liyun, Mo Ni, Jia Yunqin, Liu Min, Pu Shixue

2015, 14(4):  53-56. 

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Objective: To analyze the preschool children with ocular trauma in Dali region and put forward effective protective
measures. Methods: Twenty-seven cases with ocular trauma admitted from January 2012 to April 2014 were selected. Such factors of their ocular trauma were retrospectively analyzed as their age, gender, accident site, the cause of the accident, the nature of the injury, and prognosis. Results: Injury happened in boys more than girls, home is the main location of accident, children from five years onward were most likely to be injured in the eye, the prognosis were associated with trauma score. Conclusion: Prevention could reduce the risk of children's ocular trauma and effectively decrease the number of children with low vision.

Clinical Value of Prospective Ecg-gated CT and CarekV Technology of Coronary Artery Angiography

Li Dezhi, Yang Haiyan, Duan Yunxue, Liu Haimei, Zhang Lifang

2015, 14(4):  57-59. 

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Objective: To analyze and conclude the clinical value of prospective ECG-gated CT and carekV technology of coronary
artery angiography. Methods: Eighty cases of coronary CT angiography image data were retrospectively analyzed. Forty cases were prospective ECG-gated and CarekV technology scanning group, the other 40 cases were retrospective scanning group. Both groups were conducted ECG automatic current modulation technique.With the double-blinded method, the image quality of all segmental coronary （diameter≥1 mm） and radiation dose in two groups were evaluated. Results: The individual total radiation dose in retrospective scan group was 1.862 mSv to 9.772 mSv, the average was 5.47 mSv. The individual total radiation dose in prospective ECG-gated and CarekV technology scanning group was 0.862 mSv to 2.996 mSv, the average was 1.27 mSv. There were 576 segments obtained in retrospective scanning group, in which 97.82% achieved diagnostic criteria（≤3 points）, and there were 90.66% reached the excellent standard. In prospective ECG-gated and CarekV technology scanning group, there were 543 segments obtained, the rate of achieved diagnosis criteria was 95.44%, and 89.57% reached the excellent standard. Conclusion: The prospective ECG-gated CT and carekV technology in coronary CT angiography could provide excellent image which acheived clinical diagnostic standard and lower radiation dose.

Comparison of Two Pretreatment Methods for Determination of the Content of 6 Metal Elements in
Rapeseed Oil

Tang Guanghua, Li E, Liu Chongmei, Shi Jiao, Zhu Youshuai, Pu Shaoqun, Song Zhen

2015, 14(4):  60-62. 

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Objective: To compare the effects of different pretreatment methods on determination of the content of 6 metal elements in
rapeseed oil. Methods: 15 samples of rapeseed oil were pretreated by dry ashing and wet digestion methods and the content of 6 kinds of metal elements such as lead, cadmium, cuprum and iron was determined by flame atomic absorption spectrometry（FAAS）. Results: After dry ashing and wet digestion treatments, the difference in the content of Pb and Cu had no statistical significance（P >0.05）, but there was statistical significance in the difference in the content of Fe and Mn（P < 0.05）. R2 of each element was over 0.99. The recovery rates of standard addition with the dry ashing method and with the wet digestion method were 81.90%-107.45% and 99.90%-108.05% respectively and satisfied the requirements of trace analysis. By contrast, the difference in recovery rates of standard addition between dry ashing and wet digestion methods was statistically significant（P < 0.05）and the recovery rate of the method of wet digestion and FAAS was higher than that of the dry ashing method. Conclusion: The accurate measuring result could be obtained by both methods but the wet digestion method was superior to the dry ashing method.

Investigation of the Physical Development and Dietary Health Habits of Pupils in Some Primary
School in Dali

Ding Chunli, Dong Chengyan, Wang Wenxian, Zheng Xiaoxia, Yang Fuyou, Ji Shoulian

2015, 14(4):  63-65. 

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Objective: To survey the physical development and dietary health habits of pupils in some primary school in Dali.Methods: Total 1 121 pupil from fourth grade to sixth grade in ten different primary schools in Dali were selected for questionnaire
survey on their dietary health habits. Meanwhile, pupil's height and weight were measured. Results: 78.14% pupils were lean. The
BMI of pupils from grade four, five and six were compared and the difference was statistically significant（P < 0.01）, and the BMI of
sixth grade is greater than that of the fifth grade whose BMI was greater than that of the fourth grade. The BMI of boys and girls were compared and the difference was statistically significant（P < 0.01）. The BMI of boys was higher than that of girls. The BMI of Han,Bai, Yi, Hui, and other ethnic minorities were also compared, the difference was not statistically significant（P > 0.05）. The situation of pupils eating breakfast is better, while the situation of picky eating and eating snacks rather than meals are general. Conclusion: Most primary school students are lean and have harmful dietary health habits in Dali. To promote pupil's physical development, targeted health education should be carried out, nutrition and hygiene should be popularized.

Application of QCC in the Patient's Awareness of Preoperative Instruction in Selective Operation

Chen Xianqin, Zhang Zeyu, Dai Hongju, Xu Wei, Wang Ping, Liu Shanshan

2015, 14(4):  66-68. 

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Objective: To improve the awareness rate of preoperative instruction for gastrointestinal operation. Methods: Quality
control circle（QCC）team members applying brainstorming put forward 15 pieces of related preoperative instruction that patients
should know. Forty- six patients were conducted anonymous questionnaire. Five most unknown questions were selected and
corresponding nursing was set. Then similar patients were conducted the same questionnaire. Results: The awareness rate of
preoperative instruction was highly increased, from 80.2% to 97.1%. Conclusion: QCC management could locate problems timely and accurately. Accordingly standardized process which motivates nursing work and improves serving quality would be formed eventually.

Psychological Factors of Surgery Patients' Families at the Waiting Area and Its Interventions

Zhang Qinfei, Zhao Yuan

2015, 14(4):  69-71. 

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Objective: To discuss the psychological factors of surgery patient's families at the waiting area and its interventions.
Methods: Clinical nursing was practiced and relevant literature was analyzed and concluded. Results: Psychological factors and
interventions include: detailed understanding of patient's condition and operative risk, informing of the surgical environment and
intraoperative condition, improvement of the psychological intervention for emergency operation patient's families, health education for different education levels, conducting the most economical and effective treatment, improving medical skills and ethics. Conclusion:Effective psychological intervention could reduce the anxiety of patient's family, improve doctor-patient relationship and medical quality.

Application of Automatic Fill-prescription Devices in Inpatient Dispensary in the Affiliated Hospital of
Dali University in Chuxiong

Chen Mo, Yang Xiongchang, Xu Yaohui

2015, 14(4):  72-74. 

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Objective: To discuss the value of automatic fill-prescription devices in pharmacy. Methods: The structure, application
of automatic fill-prescription device were introduced, the advantages and disadvantages were concluded. Results: Automatic fillprescription device is suitable for inpatient dispensary. It could improve the medical quality. Conclusion: Automatic dispensing is a
trend for future pharmacy.

Health Education on Children Convulsions

Zhang Xiaolan，Zhao Yuan

2015, 14(4):  75-78. 

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Objective: To analyze and summarize the health education methods and content of children convulsions. Methods: The
health education methods and content of children convulsion were proposed by consulting literature and summarizing clinical and
teaching experience. Results：Health education methods are mainly in the form of oral language, written language, pictures, videos,
behaviors, post-discharge telephone counseling and regular follow-up visits to outpatients. The content of health education is divided into education during and after the hospitalization, including explanation of the disease, first aid measures, psychological nursing,observation of the disease and discharge guidance. Conclusion：Good health education for older children and their family can control onvulsions in time and prevent the recurrence effectively, and it also plays an important role in reducing brain injury.

Influence of the Responsibility System for Holistic Nursing Care for Hospital Left-behind Children

Zhong Wen

2015, 14(4):  79-81. 

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Objective: To evaluate the effect of the responsibility system for holistic nursing care for hospital left-behind children.
Methods: By evaluating the psychological status of hospital left-behind children, this research aims at exploring the effects of the
responsibility system for the overall care for left-behind children in southwest frontier minority settlements, including psychological
counseling, health education and behavior intervention. Results: Through the functional assessment of hospital left-behind children, it is shown that there was no statistically significant difference between the scores of two groups of children（P > 0.05）before nursingintervention; after nursing intervention, the total score for the non- nursing intervention group is 113 points, while the nursingintervened group is 336 points, which shows that the difference is statistically significant（P = 0.025 < 0.05）. Conclusion: Through high-quality nursing activities, the "people-oriented" responsibility system of the overall care can actively guide and eliminate the psychological barriers of hospital left-behind children so that a speedy recovery and health recuperation could be expected.

Noble Medical Ethics, Excellent Medical Skill and Good Management: Reflections on the Style
Construction of Hospital

Jiang Mei, Zhu Xiong, Hong Yinxiu

2015, 14(4):  82-85. 

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In the process of building a well-off society, hospital becomes the safeguard of people's health, the boost of economic
development and the link between government and citizens. As malpractice and medical dispute become rampant, the style
construction of hospital increasingly attracts people's attention. The article stresses noble medical ethics, excellent medical skills and good management are the breakthrough of hospital style construction.

Discussion Course Practice of the "Basic and Clinical Study of Nervous System Diseases"

Zhu Jianhua, Wang Yong, Yang Xinwen

2015, 14(4):  86-88. 

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Nervous system is the most complex system in human anatomy. To help students understand and apply the anatomy of
nervous system, the discussion course of "basic and clinical study of nervous system diseases" was open. This article introduced the teaching methods, advantages and disadvantages of the course, and pointed out the irreplaceable role of the course on students' learning ability, thinking ability and level of the clinical diagnosis and treatment. The course is worth of popularization in a certain range.

Exploration of Applying Inquiry and Cooperative Learning
Strategies in Pharmacy Teaching Practice

2015, 14(4):  89-92. 

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Various methods are applied in pharmacy teaching. To improve the quality of pharmacy teaching and accumulate teaching
experience, in the process of teaching practice the current content of experiment teaching is considered, inquiry-based and groupcooperative
learning strategies are developed. This article describes the features, conditions and effects of inquiry and cooperative
learning .